低蛋白氨基酸平衡日粮添加半胱胺对生长猪肉质和相关基因表达的影响

发布时间：2018-08-28 16:23

【摘要】：旨在研究低蛋白氨基酸平衡日粮添加半胱胺对生长猪肉质和相关基因表达的影响。试验采用2×2因子试验设计,选取(42.18±0.70)kg左右的杜×长×梅阉公猪120头,随机分为4处理,每个处理5个重复,每个重复6头猪,日粮蛋白水平为16%和12%,半胱胺添加水平为0和100mg·kg-1。试验期为31d。结果表明,低蛋白氨基酸平衡日粮显著降低了生长猪背最长肌的剪切力值(P0.05),显著增加了肌内脂肪的含量(P0.05),上调了固醇调节元件结合蛋白1c(SREBP-1c)、脂肪酸合成酶(FAS)、硬脂酰辅酶A脱氢酶(SCD)、μ-钙蛋白酶的mRNA表达量(P0.05),下调了激素敏感脂酶(HSL)、肉碱脂酰转移酶1(CPT-1)、钙蛋白酶抑制蛋白的mRNA表达量(P0.05);半胱胺的添加下调了钙蛋白酶抑制蛋白的mRNA表达量(P0.05)。日粮蛋白水平与半胱胺添加之间不存在交互作用(P0.05)。结果提示,低蛋白氨基酸平衡日粮可能通过上调脂质合成相关基因的表达,下调脂质降解相关基因的表达来增加肌内脂肪含量,进而改善生长猪背最长肌嫩度,半胱胺的添加对生长猪肉品质未见明显影响。
[Abstract]:The aim of this study was to study the effects of cysteamine supplementation on the quality and related gene expression of growing pork in a low protein amino acid balanced diet. A 2 脳 2 factor design was used. 120 DU 脳 Chang 脳 Mei castrated boars with (42.18 卤0.70) kg were randomly divided into 4 treatments with 5 replicates each, with dietary protein levels of 16% and 12%, cysteamine supplementation level 0 and 100mg kg-1.. The trial period was 31 days. The results show that Low protein amino acid balance diet significantly decreased the shear stress of longissimus dorsi muscle (P0.05), increased the content of intramuscular fat (P0.05), up-regulated steroid regulatory element binding protein 1c (SREBP-1c), fatty acid synthase (FAS), stearyl coenzyme A dehydrogenation. The expression of mRNA of (SCD), 渭 -cadherase (P0.05) down-regulated the expression of steroid-sensitive lipase (HSL), carnitinyltransferase 1 (CPT-1) and the mRNA expression of calpain inhibitor protein (P0.05); the addition of cysteamine down-regulated the mRNA expression of calpain inhibitor protein (P0.05). There was no interaction between dietary protein levels and cysteamine supplementation (P0.05). The results suggested that low protein amino acid balance diet may increase the content of intramuscular fat by up-regulating the expression of genes related to lipid synthesis and down-regulating the expression of genes related to lipid degradation, thereby improving the tenderness of the longissimus dorsi muscle in growing pigs. The addition of cysteamine had no significant effect on the quality of growing pork.
【作者单位】：南京农业大学动物科技学院江苏省动物源食品生产与安全保障重点实验室江苏省肉类生产与加工质量安全控制协同创新中心;
【基金】：国家重点基础研究发展计划课题(973计划)(2013CB127306)
【分类号】：S828.5

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