不同来源金黄色葡萄球菌的肠毒素基因分布与SCCmec，spa及MLST分型研究
发布时间：2018-08-31 11:59
【摘要】：本研究收集了496株金黄色葡萄球菌(S. aureus),菌株来源涉及人源、器械源、动物源和食品源。根据文献报道的18种S. aureus肠毒素基因(sea-see, seg～sei, ser)及类肠毒素基因(selj-selq, selu)设计引物,建立了多重PCR检测方法并利用该方法检测了496个S. aureus菌株中肠毒素基因及类肠毒素基因的分布。其中,34.27%的菌株携带经典型肠毒素基因(sea-see),27.02%的菌株携带新型肠毒素基因(seg-sei, ser)及42.94%的菌株携带类肠毒素基因(selj-selq, selu)。检测率最高的肠毒素基因分别为sea (19.76%), seg (19.15%), selo (19.15%), selm (16.94%), seln (16.33%)及sei(15.92%)。同时发现了两个新的肠毒素基因簇,即基因簇(seg-selo-selm-seln-sei)与基因簇(seg-selo-selm-seln-sei-selu)。另外,一些肠毒素及类肠毒素基因常出现在同一菌株中,例如：ser, sed, selj; sea, selk, selq; selk, selq。携带seh基因的金黄色葡萄球菌均为MSSA菌株。在本研究中,新型肠毒素及类肠毒素基因在不同来源的金黄色葡萄球菌中的出现率很高,由此可见,金黄色葡萄球菌肠毒素基因的检测不能局限于5种经典型肠毒素基因,新型肠毒素基因及类肠毒素基因应引起重视。本研究对192株金黄色葡萄球菌通过spa及MLST分型技术进行分型,并对66株MRSA进行SCCmec分型,共发现45种ST型,59种spa型及5种SCCmec型。人源MRSA主要为CC239-t030-SCCmec III. CC239-t037-SCCmec Ⅲ、ST630-t4549-SCCmec V与ST630-t2196-SCCmec V型；器械源MRSA几乎均为ST630-t4549-SCCmec V型；牛源MRSA均为ST630-t2196-SCCmecⅤ型；猪源MRSA均分布于CC9,而未能进行SCCmec分型的猪源MRSA均为ST9-t899与ST2630-t1939型。Sea-selk-selq、selk-selq及sea-selq在CC239-SCCmec III型MRSA菌株中有着广泛分布,CC9的MRSA菌株中有selk-selq的分布,而sea-selk-selq在CC5的MSSA菌株中同样存在。Seg-sei-selm-seln-selo在CC5-SCCmec Ⅱ型MRSA及seg-sei-selm-seln-selo-selu在CC1301-SCCmec Ⅴ型MRSA中均有分布,而这两种基因簇更多的分布于CC5、CC25、CC30、CC45及CC1301的MSSA菌株中。研究表明某些肠毒素基因尤其是肠毒素基因簇存在于一些特殊基因型的菌株中,而且可能和MRSA有着关联。
[Abstract]:In this study, 496 Staphylococcus aureus (S. aureus), strains were collected from human, instrument, animal and food sources. Based on the primer design of 18 kinds of S. aureus enterotoxin genes (sea-see, seg~sei, ser) and enterotoxin-like genes (selj-selq, selu) reported in the literature, a multiplex PCR method was established to detect the distribution of enterotoxigenic genes and enterotoxigenic genes in 496 S. aureus strains. Of these, 34.27% of the strains carried classical enterotoxin gene (sea-see) and 27.02% of the strains carried a novel enterotoxin gene (seg-sei, ser) and 42.94% of the strains carried the enterotoxin-like gene (selj-selq, selu). The highest detection rate of enterotoxin gene was sea (19.76%), seg (19.15%), selo (19.15%), selm (16.94%), seln (16.33%) and sei (15.92%). At the same time, two new enterotoxin gene clusters were found, namely gene cluster (seg-selo-selm-seln-sei) and gene cluster (seg-selo-selm-seln-sei-selu). In addition, some enterotoxigenic and enterotoxigenic genes often appear in the same strain, such as: ser, sed, selj; sea, selk, selq; selk, selq. Staphylococcus aureus carrying seh gene were all MSSA strains. In this study, the presence of new enterotoxin and enterotoxin-like genes in Staphylococcus aureus from different sources is very high. Therefore, the detection of Staphylococcus aureus enterotoxin gene can not be limited to five classical enterotoxin genes. New enterotoxin genes and enterotoxigenic genes should be paid attention to. In this study, 192 strains of Staphylococcus aureus were classified by spa and MLST typing techniques, and 66 strains of MRSA were genotyped by SCCmec. A total of 45 ST types 59 spa types and 5 SCCmec types were found. Human MRSA is mainly CC239-t030-SCCmec III. CC239-t037-SCCmec 鈪，
本文编号：2214919
[Abstract]:In this study, 496 Staphylococcus aureus (S. aureus), strains were collected from human, instrument, animal and food sources. Based on the primer design of 18 kinds of S. aureus enterotoxin genes (sea-see, seg~sei, ser) and enterotoxin-like genes (selj-selq, selu) reported in the literature, a multiplex PCR method was established to detect the distribution of enterotoxigenic genes and enterotoxigenic genes in 496 S. aureus strains. Of these, 34.27% of the strains carried classical enterotoxin gene (sea-see) and 27.02% of the strains carried a novel enterotoxin gene (seg-sei, ser) and 42.94% of the strains carried the enterotoxin-like gene (selj-selq, selu). The highest detection rate of enterotoxin gene was sea (19.76%), seg (19.15%), selo (19.15%), selm (16.94%), seln (16.33%) and sei (15.92%). At the same time, two new enterotoxin gene clusters were found, namely gene cluster (seg-selo-selm-seln-sei) and gene cluster (seg-selo-selm-seln-sei-selu). In addition, some enterotoxigenic and enterotoxigenic genes often appear in the same strain, such as: ser, sed, selj; sea, selk, selq; selk, selq. Staphylococcus aureus carrying seh gene were all MSSA strains. In this study, the presence of new enterotoxin and enterotoxin-like genes in Staphylococcus aureus from different sources is very high. Therefore, the detection of Staphylococcus aureus enterotoxin gene can not be limited to five classical enterotoxin genes. New enterotoxin genes and enterotoxigenic genes should be paid attention to. In this study, 192 strains of Staphylococcus aureus were classified by spa and MLST typing techniques, and 66 strains of MRSA were genotyped by SCCmec. A total of 45 ST types 59 spa types and 5 SCCmec types were found. Human MRSA is mainly CC239-t030-SCCmec III. CC239-t037-SCCmec 鈪，
本文编号：2214919
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