奶牛源MRSA流行株的耐药特性SCCmecA分型研究

发布时间：2018-09-01 11:13

【摘要】：为了了解引起奶牛乳房炎的金黄色葡萄球菌分离株的耐药性及其分子流行病学特征,本研究采用纸片扩散法检测临床分离的71株金黄色葡萄球菌的耐药特性,并对耐甲氧西林金黄色葡萄球菌(Methicillin-resistant staphylococcus aureus,MRSA)进行判定;应用多重PCR方法对MRSA进行SCCmec分型。结果显示:在新疆地区71株金黄色葡萄球菌临床分离株中共检出13株MRSA,检出率为18.3%;金黄色葡萄球菌对呋喃妥因、利福平100%敏感,MRSA对头孢类、氟喹诺酮类和大环内酯类抗菌药物的耐药率明显高于甲氧西林敏感金黄色葡萄球菌(methicillin sensitive staphylococcus aureus,MSSA);MRSA的SCCmec基因型共以SCCmecⅠ和SCCmecⅣa型为主,均占76.9%,另外,1株MRSA仅有1种SCCmecⅢ型,1株MRSA有SCCmecⅠ、SCCmecⅢ和SCCmecⅣa型。本研究结果表明,MRSA耐药性多样,新疆地区MRSA主要流行株为SCCmecⅠ和SCCmecⅣa型。
[Abstract]:In order to understand the drug resistance and molecular epidemiological characteristics of Staphylococcus aureus isolates causing mastitis in dairy cattle, the drug resistance of 71 clinical isolates of Staphylococcus aureus was detected by disk diffusion method. Methicillin-resistant Staphylococcus aureus (Methicillin-resistant staphylococcus aureus,MRSA) was identified and SCCmec typing of MRSA was performed by multiple PCR method. The results showed that 13 strains of MRSA, were detected in 71 clinical isolates of Staphylococcus aureus in Xinjiang area, the sensitivity of Staphylococcus aureus to furantoin and rifampicin was 100% to cephalosporins, and the positive rate of Staphylococcus aureus to furantoin and rifampicin was 100%. The resistant rates of fluoroquinolones and macrolides were significantly higher than those of methicillin-sensitive Staphylococcus aureus (methicillin sensitive staphylococcus aureus,MSSA). The SCCmec genotypes of MRSA were mainly SCCmec 鈪，

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