体外消化法优化生长猪玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮非淀粉多糖酶谱的研究

发布时间：2018-09-03 15:11

【摘要】：旨在探讨使用体外法模拟猪饲粮胃肠道消化,优化生长猪玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮的非淀粉多糖(NSP)酶谱。试验首先采用单因素完全随机设计,在玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮中分别添加不同水平的单一NSP酶:纤维素酶、木聚糖酶、β-甘露聚糖酶、α-半乳糖苷酶、β-葡聚糖酶和果胶酶,采用基于生长猪生理消化的模拟胃液-小肠液体外法对饲粮体外干物质消化率(in vitro dry matter digestibility,IVDMD)进行测定,分析各NSP酶对饲粮IVDMD的作用效果。然后采用二次回归通用旋转设计,分别建立两种饲粮IVDMD与6种NSP酶的回归关系,筛选两种饲粮中6种NSP酶的最佳组合,再对优化的酶谱组合进行体外验证。结果显示:1)生长猪玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮的IVDMD与6种NSP酶的添加水平之间均存在二次曲线关系;2)在本试验条件下,玉米-豆粕-DDGS饲粮的最佳酶谱:纤维素酶427.1U·kg~(-1)、木聚糖酶7 057.5U·kg~(-1)、β-甘露聚糖酶3 587.8U·kg~(-1)、α-半乳糖苷酶202.1U·kg~(-1)、β-葡聚糖酶1 543.3U·kg~(-1)和果胶酶72.7U·kg~(-1)。小麦-豆粕饲粮的最佳酶谱:纤维素酶1 117.9 U·kg~(-1)、木聚糖酶35 087.7 U·kg~(-1)、β-甘露聚糖酶1 917.1U·kg~(-1)、α-半乳糖苷酶305.0U·kg~(-1)、β-葡聚糖酶806.7U·kg~(-1)和果胶酶133.7U·kg~(-1);3)优化后的NSP酶谱组合使玉米-豆粕-DDGS饲粮的IVDMD提升了3.89%;使小麦-豆粕饲粮的IVDMD提升了3.48%。结果表明,体外法优化后的NSP酶谱组合和配伍能更大程度地提高生长猪玉米-豆粕-DDGS饲粮和小麦-豆粕饲粮的IVDMD,基于生长猪生理消化的模拟胃液-小肠液体外消化法可快速筛选出生长猪饲粮合理的NSP酶谱。
[Abstract]:The aim of this study was to optimize the (NSP) zymogram of non-starch polysaccharides (NPS) in growing porcine corn-soybean dregs and wheat-soybean meal diets by simulating the digestion of gastrointestinal tract in pig diets in vitro. In the experiment, a single NSP enzyme, cellulase, was added to corn-soybean meal diet and wheat-soybean meal diet, respectively. Xylanase, 尾 -mannanase, 伪 -galactosidase, 尾 -glucanase and Pectinase were used to determine the dry matter digestibility (in vitro dry matter digestibility,IVDMD) of diet in vitro by simulated gastric liquid-small intestinal fluid method based on physiological digestion of growing pigs. The effects of NSP enzymes on dietary IVDMD were analyzed. Then the quadratic regression general rotation design was used to establish the regression relationship between two dietary IVDMD and six NSP enzymes, and the optimal combination of six NSP enzymes in the two diets was selected, and the optimized zymogram combination was verified in vitro. The results showed that there was a conic relationship between the levels of IVDMD and six NSP enzymes in the corn-soybean dregs and wheat-soybean meal diets of growing pigs. The optimum zymogram of corn-soybean dregs diet: cellulase 427.1 U kg~ (-1), xylanase 7 057.5 U kg~ (-1), 尾 -mannanase 3 587.8 U kg~ (-1), 伪 -galactosidase 202.1 U kg~ (-1), 尾 -glucanase 1 543.3 U kg~ (-1) and Pectinase 72.7 U kg~ (-1). Optimum zymogram of wheat-soybean meal diet: cellulase 1 117.9 U kg~ (-1), xylanase 35 087.7 U kg~ (-1), 尾 -mannanase 1 917.1 U kg~ (-1), 伪 -galactosidase 305.0 U kg~ (-1), 尾 -glucanase 806.7 U kg~ (-1) and Pectinase 133.7 U kg~ (-1) optimized NSP zymogram combination of corn-soybean dregs-DDGS diet IVDMD The IVDMD of wheat-soybean meal diet was increased by 3. 48%. The results show that The combination and compatibility of NSP zymogram optimized by in vitro method can greatly improve the IVDMD, of growing pig corn-soybean dregs and wheat-soybean meal diets based on the simulated gastric juice and intestinal fluid extradigestion method based on physiological digestion of growing pigs. The reasonable NSP zymogram of growing pig diet was screened out.
【作者单位】：中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室;广东温氏食品集团股份有限公司;
【基金】：中央级公益性科研院所基本科研业务费专项(2017ywf-zd-18) 中国农业科学院科技创新工程(ASTIP-IAS07)
【分类号】：S828.5

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