禽波氏菌强毒株感染雏鸡呼吸系统荷菌量及病变规律的研究

发布时间：2018-09-04 14:09

【摘要】：禽波氏菌为产碱杆菌科波氏杆菌属成员,主要导致咳嗽等呼吸道症状。为探索禽波氏菌经呼吸道的入侵过程及其病变发生发展规律,本试验利用筛选到的强毒株经鼻腔感染SPF雏鸡,在感染后的不同时间检测气管和肺荷菌量的变化,通过扫描电镜观察禽波氏菌在气管的定植及其病变规律,利用间接免疫酶组化研究禽波氏菌在肺中的定植规律,分析肺和支气管的病理组织学变化。结果发现,在感染之初气管中细菌数量较多,而肺中较少,之后气管和肺的荷菌量均呈明显上升趋势。感染后1d即发现禽波氏菌已经在气管纤毛上定植,且纤毛开始出现脱落;到5d时气管黏膜表面呈斑驳状,纤毛大面积脱落,甚至出现空洞。肺在感染后2d检测出细菌定植,主要分布在肺房和呼吸毛细管壁的细胞质中,之后随着感染时间的延长,阳性信号不断增加。肺和支气管的病理变化在5d时出现,主要表现淤血;至感染后10d,大量细胞坏死。试验结果表明所筛选禽波氏菌分离株对雏鸡的呼吸道具有很强的亲嗜性,优先定植于气管上皮细胞的纤毛上,并随之在气管、支气管和肺产生一系列的组织病理学变化。
[Abstract]:Pasteurella fowls is a member of Alcaligenes Coppolis, which mainly causes respiratory symptoms such as cough. In order to explore the invasion process and the development of the disease in the respiratory tract, the virulent strains were used to infect SPF chicks through nasal cavity, and the changes of trachea and pulmonary bacillus were detected at different time after infection. By means of scanning electron microscope, the colonization and pathological changes of Bordetella fowls in the trachea were observed. The colonization of Botryella fowlorii in the lung was studied by indirect immunohistochemistry, and the histopathological changes of the lungs and bronchi were analyzed. The results showed that there were more bacteria in trachea and less in lung at the beginning of infection, and then the amount of bacteria in trachea and lung increased obviously. One day after infection, it was found that Botrytis fowls had been colonized on the tracheal cilia, and the cilia began to fall off, and on the 5th day, the surface of the tracheal mucosa was mottled, the cilia fell off in a large area, and even the cavity appeared. Bacterial colonization was detected in the lung 2 days after infection, mainly in the cytoplasm of pulmonary atrium and respiratory capillary wall, and the positive signal increased with the extension of infection time. The pathological changes of lung and bronchus appeared at 5 days, mainly showed congestion, and at 10 days after infection, a large number of cells were necrotic. The results showed that the isolated strains had a strong affinity to the respiratory tract of chicks, and were preferentially located on the cilia of tracheal epithelial cells, with a series of histopathological changes in trachea, bronchus and lung.
【作者单位】：山东农业大学动物科技学院;山东省动物疫病预防与控制中心;
【基金】：国家自然科学基金(31602067;31272595) 山东省自然科学基金(ZR2014CM044)
【分类号】：S858.31

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