梅花猪胃肠道5-羟色胺、胃饥饿素和胰高血糖素样肽-1免疫阳性细胞分布特征研究
发布时间：2018-09-06 15:24
【摘要】：为了解中国优良地方猪种梅花猪胃肠道内分泌细胞的分布与形态特征,本试验选取6头30日龄断奶健康梅花仔猪,在相同条件下饲养至65日龄,所有仔猪饲喂相同日粮,自由采食饮水。试验结束时,收集仔猪胃肠道各段组织样品,应用免疫组织化学方法观察3种胃肠道内分泌激素5-羟色胺(5-HT)、胃饥饿素(ghrelin)和胰高血糖素样肽-1(GLP-1)免疫阳性细胞的分布及形态特征,并对阳性细胞密度进行分析。结果显示,梅花猪的肠道不同部位(十二指肠、空肠、回肠和结肠)均存在5-HT阳性细胞,主要位于肠上皮组织中,呈锥形、圆形、梭形等形态,可分为开放型和闭合型,其中十二指阳性细胞肠密度显著高于其他肠段(P0.05);梅花猪的胃肠道不同部位均有Ghrelin阳性细胞,阳性细胞密度也是随胃肠道走向递减,胃腺体部密度最高;梅花猪的肠道不同部位均有GLP-1阳性细胞,阳性细胞密度沿肠道走向呈抛物线分布,其中回肠数量最多。本试验结果为进一步探究梅花猪胃肠道生理奠定了基础。
[Abstract]:In order to understand the distribution and morphological characteristics of endocrine cells in gastrointestinal tract of China's fine local pig breeds, six 30 day weaned healthy Plum Piglet piglets were selected and fed to 65 days of age under the same conditions. All piglets were fed the same diet. Free drinking and drinking water. At the end of the experiment, the distribution and morphological characteristics of immunoreactive cells of three gastrointestinal endocrine hormones (5-HT, (ghrelin) and GLP-1) in piglets were observed by immunohistochemical method. The density of positive cells was analyzed. The results showed that there were 5-HT positive cells in different parts of the intestine (duodenum, jejunum, ileum and colon) of the pig, mainly located in the intestinal epithelium, which were conical, round and fusiform, and could be divided into open type and closed type. The intestinal density of positive cells in 12 fingers was significantly higher than that in other intestinal segments (P0.05), and Ghrelin positive cells were found in different parts of the gastrointestinal tract, and the density of positive cells decreased with the trend of gastrointestinal tract, and the density of gastric glands was the highest. GLP-1 positive cells were found in different parts of the intestinal tract of the pig, and the density of the positive cells was parabola along the direction of the intestine, and the number of the ileum was the most. The results of this study laid a foundation for further exploring the gastrointestinal physiology of Plum Plum Pigs.
【作者单位】： 广东省农业科学院农业生物基因研究中心;
【基金】：广东省主体科研机构创新能力建设专项“华南农业基因研究与创新平台建设”项目(2011070703) 广东省科技计划项目(2013B020306004、2016B070701013) 广东省农业科学院院长基金项目(201220)
【分类号】：S828
，
本文编号：2226767
[Abstract]:In order to understand the distribution and morphological characteristics of endocrine cells in gastrointestinal tract of China's fine local pig breeds, six 30 day weaned healthy Plum Piglet piglets were selected and fed to 65 days of age under the same conditions. All piglets were fed the same diet. Free drinking and drinking water. At the end of the experiment, the distribution and morphological characteristics of immunoreactive cells of three gastrointestinal endocrine hormones (5-HT, (ghrelin) and GLP-1) in piglets were observed by immunohistochemical method. The density of positive cells was analyzed. The results showed that there were 5-HT positive cells in different parts of the intestine (duodenum, jejunum, ileum and colon) of the pig, mainly located in the intestinal epithelium, which were conical, round and fusiform, and could be divided into open type and closed type. The intestinal density of positive cells in 12 fingers was significantly higher than that in other intestinal segments (P0.05), and Ghrelin positive cells were found in different parts of the gastrointestinal tract, and the density of positive cells decreased with the trend of gastrointestinal tract, and the density of gastric glands was the highest. GLP-1 positive cells were found in different parts of the intestinal tract of the pig, and the density of the positive cells was parabola along the direction of the intestine, and the number of the ileum was the most. The results of this study laid a foundation for further exploring the gastrointestinal physiology of Plum Plum Pigs.
【作者单位】： 广东省农业科学院农业生物基因研究中心;
【基金】：广东省主体科研机构创新能力建设专项“华南农业基因研究与创新平台建设”项目(2011070703) 广东省科技计划项目(2013B020306004、2016B070701013) 广东省农业科学院院长基金项目(201220)
【分类号】：S828
，
本文编号：2226767
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