离子排斥色谱法测定瘤胃液中乳酸和挥发性脂肪酸的含量
发布时间：2018-09-10 05:58
【摘要】：为了研究反刍动物瘤胃液内各有机酸含量水平,建立同时测定瘤胃液中乳酸和6种挥发性脂肪酸的高效液相色谱分析方法,试验采用离子排斥色谱模式,采用Sepax Carbomix H-NP 5(300×7.8 mm ID,5μm,8%交联度)色谱柱,配同系保护柱,柱温55℃,流动相采用2.5 mmol/L H2SO4水溶液,流速0.50 m L/min,示差折光检测器温度30℃,响应时间6 s。结果表明:乳酸和6种挥发性脂肪酸30 min内基线分离无干扰,在5.0~200.0 mg/L浓度范围内呈现良好的线性关系,各组分方法检测限在0.24~0.46 mg/L之间。说明该方法样品前处理简便,重现性好,结果准确。
[Abstract]:In order to study the content of organic acids in rumen fluid of ruminant, a high performance liquid chromatography (HPLC) method for simultaneous determination of lactic acid and 6 volatile fatty acids in rumen fluid was established. Sepax Carbomix H-NP _ 5 (300 脳 7.8 mm ID,5 渭 m ~ (8%) crosslinking degree) column was used, the column temperature was 55 鈩，
本文编号：2233563
[Abstract]:In order to study the content of organic acids in rumen fluid of ruminant, a high performance liquid chromatography (HPLC) method for simultaneous determination of lactic acid and 6 volatile fatty acids in rumen fluid was established. Sepax Carbomix H-NP _ 5 (300 脳 7.8 mm ID,5 渭 m ~ (8%) crosslinking degree) column was used, the column temperature was 55 鈩，
本文编号：2233563
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