不同长度的聚肌胞对SPF鸡固有免疫增强作用的研究

发布时间：2018-09-10 10:56

【摘要】：聚肌胞(poly I:C,p IC)作为强干扰素诱生剂,在人类医学上被用作抗病毒和抗肿瘤药物。已有的研究表明,聚合长度不同的p IC对哺乳动物细胞的免疫增强作用不同。为明确p IC的聚合长度是否会影响其对鸡的固有免疫增强作用,本研究将低分子量p IC(0.3 kb~1 kb)和高分子量p IC(6 kb~8 kb)分别肌肉注射1月龄的SPF鸡,各设置2个p IC浓度梯度。在p IC作用后的6 h、12 h、24 h和36 h,分别采集EDTA抗凝血,分离外周血淋巴细胞(PBL),通过荧光定量PCR方法检测PBL固有免疫信号通路相关基因的转录水平变化。结果表明,低和高分子量范围的p IC刺激SPF鸡后均可以迅速激活鸡体固有免疫应答,引起TLR3、MDA5、IFN-α、IFN-β、IFN-γ、IL-1β、IL-2、IL-6和IL-17等免疫相关基因转录水平的上调。该结果与哺乳动物模型的研究报道截然不同,而与本实验室以多种鸡源细胞为模型的研究结果相似。10μg/kg(质量/体质量)的p IC即可以引起PBL固有免疫相关基因转录水平上调,而且大部分基因在p IC刺激后6 h即达到转录峰值,表明p IC作为鸡用免疫增强剂的高效性。总之,本研究表明短链和长链p IC均可用作鸡免疫增强剂。
[Abstract]:Poly I: CP IC, as a strong interferon inducer, has been used as antiviral and antitumor drugs in human medicine. Previous studies have shown that p IC with different polymerization lengths has different immune enhancement effects on mammalian cells. In order to determine whether the polymerization length of p IC would affect the inherent immune enhancement of chicken, the low molecular weight p IC (0.3 kb~1 kb) and the high molecular weight p IC (6 kb~8 kb were intramuscularly injected into 1 month old SPF chickens, each with two p IC concentration gradients. EDTA anticoagulant blood was collected at 6 h, 12 h and 36 h after treatment with p IC. (PBL), was isolated from peripheral blood lymphocytes to detect the transcriptional changes of the genes associated with the intrinsic immune signaling pathway of PBL by fluorescence quantitative PCR method. The results showed that both low and high molecular weight p IC stimulated SPF chickens' innate immune response and induced the up-regulation of TLR3,MDA5,IFN- 伪 -IFN- 尾 -IFN- 尾 -IFN- 尾 IL-2IL-6, IL-17 and so on. The results were different from those reported in mammalian models, and similar to the results of our laboratory studies using a variety of chicken source cells, p IC (mass / body mass) of .10 渭 g/kg (mass / body mass) could up-regulate the transcription level of PBL innate immune-associated genes. Most of the genes reached the peak of transcription at 6 h after p IC stimulation, indicating the high efficiency of p IC as an immunoenhancer for chicken. In conclusion, both short chain and long chain p IC can be used as immune enhancers in chickens.
【作者单位】：山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室;山东农业大学动物科技学院;
【基金】：十二五国家科技支撑计划课题(2015BAD12B03) 公益性行业(农业)科研专项(201303033) 现代农业产业技术体系建设专项资金(CARS-42-Z12)
【分类号】：S852.4

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