屎肠球菌SF68对断奶仔猪生产性能、肠道形态和微生物区系的影响
发布时间：2018-09-11 21:19
【摘要】：本试验研究了屎肠球菌SF68对断奶仔猪生产性能、腹泻情况、抗氧化性能、小肠组织形态学和肠道微生物区系的影响。1、屎肠球菌SF68对断奶仔猪生长性能、腹泻情况和抗氧化性能的影响选取90头21日龄体重相近(6.58±0.07kg)的杜洛克×长白×大约克夏去势仔猪,公母各半,随机分配至5组,每组3个重复,每个重复6头猪。5个处理组分别为:对照组(CON组),饲喂不添加抗生素和屎肠球菌的基础饲粮;抗生素组(ANT组),基础饲粮中添加0.02%的硫酸抗敌素(有效含量为10%)和0.0075%的金霉素(有效含量为15%);抗生素加低屎肠球菌组(ALEF组),基础饲粮中添加0.01%屎肠球菌(含量为1.0×1010CFU/g。)、0.02%硫酸抗敌和0.0075%金霉素;低屎肠球菌组(LEF组),基础饲粮中添加0.01%屎肠球菌;高屎肠球菌组(HEF组),基础饲粮中添加0.05%屎肠球菌。试验期21天。试验期间,记录并计算平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G),统计腹泻率和腹泻指数。在仔猪42日龄时前腔静脉采血,测定血清、肝脏、空肠和回肠抗氧化指标。结果表明:(1)饲粮中添加屎肠球菌对断奶仔猪平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)无显著影响。ALEF组仔猪腹泻率显著低于CON组(P0.05);LEF组腹泻率相比于CON组有降低的趋势(P0.1);ALEF、LEF组腹泻指数相比CON组有降低的趋势(P0.1)。(2)ALEF组和LEF组血清中H2O2含量显著高于CON组(P0.05);LEF组肝脏中H2O2含量极显著低于CON组(P0.01)。ALEF组和HEF组肝脏中SOD活力、T-AOC活力显著高于CON组(P0.05)。ALEF组、LEF组和HEF组空肠中H2O2含量显著低于CON组(P0.05)。ALEF组空肠中SOD活性显著高于CON组(P0.05)。相比于CON组,其他各组回肠中SOD活力显著升高(P0.05),且MDA含量显著降低(P0.05)。2、屎肠球菌SF68对断奶仔猪肠道组织形态的影响选取十二指肠、空肠和回肠制作光镜切片,小肠中段制作透射电镜切片。测定绒毛高度(VH)、隐窝深度(CD)并计算VH:CD,观察纹状缘厚度。结果表明:(1)LEF组十二指肠绒毛高度显著高于CON组(P0.05),ALEF组VH:CD显著高于CON组(P0.05)。ALEF组和HEF组空肠绒毛高度、VH:CD显著高于CON组(P0.05)。(2)CON组和ANT组小肠微绒毛都出现了不同程度的脱落,CON组和ANT组纹状缘厚度均明显低于其他各组。相比于CON组,ALEF组、LEF组纹状缘更为整齐、致密。3、屎肠球菌SF68对断奶仔猪肠道微生物区系的影响试验结束日(42日龄)屠宰仔猪,采集结肠内容物,采用16SrRNA高通量测序技术分析屎肠球菌SF68对断奶仔猪肠道微生物区系的影响。结果表明:(1)LEF组和HEF组Chao1指数显著大于CON组(P0.05)。(2)LEF组厚壁菌门(Firmicutes)、软壁菌门(Tenericutes)比例显著高于CON组,且纤维杆菌门(Fibrobacteres)的比例有增加的趋势(P0.1)。(3)LEF组和HEF组肠道菌群结构比较相似,在树状图中形成独立分支,与CON组Unifrac距离较远。乳酸杆菌属(Lactobacillus)在组间丰度上有显著差异(P0.05)。综上,屎肠球菌SF68可提高断奶仔猪生长性能并减少腹泻,该作用可能与其减少氧化应激并维护肠道黏膜屏障有关。屎肠球菌SF68的最适添加浓度为0.01%(1.0 ×10'0CFU/g)。
[Abstract]:The effects of Enterococcus faecalis SF68 on growth performance, diarrhea, antioxidant capacity, intestinal histomorphology and intestinal microflora of weaned piglets were studied. 1. Enterococcus faecalis SF68 on growth performance, diarrhea and antioxidant capacity of weaned piglets. 90 21-day-old piglets with similar body weight (6.58.07 kg) were selected as Duroc Large Yorkshire weaned piglets, male and female, were randomly allocated to 5 groups, 3 replicates in each group, and 6 pigs in each replicate group.5 treatment group were: control group (group CON), feeding basal diet without antibiotics and Enterococcus faecium; antibiotic group (group ANT), adding 0.02% sulphuric acid (ANT) and 0.0075% chlortetracycline in basal diet. The effective content was 15%; antibiotics plus Enterococcus faecium group (ALEF group), basic diet with 0.01% Enterococcus faecium (1.0 *1010 CFU/g.), 0.02% sulfuric acid and 0.0075% chloramphenicol; Enterococcus faecium group (LEF group), basic diet with 0.01% Enterococcus faecium; Enterococcus faecium faecium group (HEF group), basic diet with 0.05% Enterococcus faecium. The average daily gain (ADG), average daily feed intake (ADFI) and feed-to-weight ratio (F/G) were recorded and calculated. The diarrhea rate and diarrhea index were calculated. The antioxidant indexes of serum, liver, jejunum and ileum were measured at 42 days old piglets. The diarrhea rate of ALEF group was significantly lower than that of CON group (P 0.05); the diarrhea rate of LEF group was significantly lower than that of CON group (P 0.1); the diarrhea index of ALEF and LEF group was significantly lower than that of CON group (P 0.1). (2) The content of H2O2 in serum of ALEF group and LEF group was significantly higher than that of CON group (P 0.05). The activity of SOD in liver and T-AOC in ALEF group and HEF group were significantly higher than that in CON group (P 0.05). The content of H2O2 in jejunum of ALEF group, LEF group and HEF group was significantly lower than that of CON group (P 0.05). The activity of SOD in jejunum of ALEF group was significantly higher than that of CON group (P 0.05). 5) and the content of MDA decreased significantly (P 0.05). 2. Enterococcus faecium SF68 on the intestinal morphology of weaned piglets. Duodenum, jejunum and ileum were selected to make light microscopic section, and mid-small intestine was made transmission electron microscopic section. The height of villi in ALEF group and HEF group was significantly higher than that in CON group (P 0.05). The height of villi in ALEF group and HEF group was significantly higher than that in CON group (P 0.05). (2) The microvilli of small intestine in CON group and ANT group were shedding in different degrees, and the thickness of striated margin in CON group and ANT group was significantly lower than that in other groups. Enterococcus faecalis SF68 on the intestinal microflora of weaned piglets at the end of the experiment (42 days old) slaughtered piglets, collected colon contents, and analyzed the effects of Enterococcus faecalis SF68 on intestinal microflora of weaned piglets by 16S rRNA high-throughput sequencing technique. The results showed that: (1) Chao1 index of LEF and HEF groups was significant. The proportion of Firmicutes and Tenericutes in LEF group was significantly higher than that in CON group (P 0.05). (3) The structure of intestinal flora in LEF group and HEF group was similar, forming an independent branch in the dendrogram, and was far away from Unifrac in CON group. In conclusion, Enterococcus faecium SF68 can improve growth performance and reduce diarrhea in weaned piglets. This effect may be related to reducing oxidative stress and maintaining intestinal mucosal barrier. The optimum concentration of Enterococcus faecium SF68 is 0.01% (1.0 *10'0 CFU/g).
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S828.5
本文编号：2237874
[Abstract]:The effects of Enterococcus faecalis SF68 on growth performance, diarrhea, antioxidant capacity, intestinal histomorphology and intestinal microflora of weaned piglets were studied. 1. Enterococcus faecalis SF68 on growth performance, diarrhea and antioxidant capacity of weaned piglets. 90 21-day-old piglets with similar body weight (6.58.07 kg) were selected as Duroc Large Yorkshire weaned piglets, male and female, were randomly allocated to 5 groups, 3 replicates in each group, and 6 pigs in each replicate group.5 treatment group were: control group (group CON), feeding basal diet without antibiotics and Enterococcus faecium; antibiotic group (group ANT), adding 0.02% sulphuric acid (ANT) and 0.0075% chlortetracycline in basal diet. The effective content was 15%; antibiotics plus Enterococcus faecium group (ALEF group), basic diet with 0.01% Enterococcus faecium (1.0 *1010 CFU/g.), 0.02% sulfuric acid and 0.0075% chloramphenicol; Enterococcus faecium group (LEF group), basic diet with 0.01% Enterococcus faecium; Enterococcus faecium faecium group (HEF group), basic diet with 0.05% Enterococcus faecium. The average daily gain (ADG), average daily feed intake (ADFI) and feed-to-weight ratio (F/G) were recorded and calculated. The diarrhea rate and diarrhea index were calculated. The antioxidant indexes of serum, liver, jejunum and ileum were measured at 42 days old piglets. The diarrhea rate of ALEF group was significantly lower than that of CON group (P 0.05); the diarrhea rate of LEF group was significantly lower than that of CON group (P 0.1); the diarrhea index of ALEF and LEF group was significantly lower than that of CON group (P 0.1). (2) The content of H2O2 in serum of ALEF group and LEF group was significantly higher than that of CON group (P 0.05). The activity of SOD in liver and T-AOC in ALEF group and HEF group were significantly higher than that in CON group (P 0.05). The content of H2O2 in jejunum of ALEF group, LEF group and HEF group was significantly lower than that of CON group (P 0.05). The activity of SOD in jejunum of ALEF group was significantly higher than that of CON group (P 0.05). 5) and the content of MDA decreased significantly (P 0.05). 2. Enterococcus faecium SF68 on the intestinal morphology of weaned piglets. Duodenum, jejunum and ileum were selected to make light microscopic section, and mid-small intestine was made transmission electron microscopic section. The height of villi in ALEF group and HEF group was significantly higher than that in CON group (P 0.05). The height of villi in ALEF group and HEF group was significantly higher than that in CON group (P 0.05). (2) The microvilli of small intestine in CON group and ANT group were shedding in different degrees, and the thickness of striated margin in CON group and ANT group was significantly lower than that in other groups. Enterococcus faecalis SF68 on the intestinal microflora of weaned piglets at the end of the experiment (42 days old) slaughtered piglets, collected colon contents, and analyzed the effects of Enterococcus faecalis SF68 on intestinal microflora of weaned piglets by 16S rRNA high-throughput sequencing technique. The results showed that: (1) Chao1 index of LEF and HEF groups was significant. The proportion of Firmicutes and Tenericutes in LEF group was significantly higher than that in CON group (P 0.05). (3) The structure of intestinal flora in LEF group and HEF group was similar, forming an independent branch in the dendrogram, and was far away from Unifrac in CON group. In conclusion, Enterococcus faecium SF68 can improve growth performance and reduce diarrhea in weaned piglets. This effect may be related to reducing oxidative stress and maintaining intestinal mucosal barrier. The optimum concentration of Enterococcus faecium SF68 is 0.01% (1.0 *10'0 CFU/g).
【学位授予单位】：扬州大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S828.5
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