山东省貂源铜绿假单胞菌分离株的血清型和致病性
发布时间：2018-09-12 15:49
【摘要】：为了解山东省貂源铜绿假单胞菌(PA)的血清型分布,本研究从部分市县采集的山东77份水貂肺脏病料样品中分离PA,并进行生化鉴定、分子生物学鉴定、血清学鉴定、生长曲线测定和半数致死量(LD_(50))测定。结果显示,共分离获得53株PA,分别属于11个血清型,其中G型27株、B型5株、D型4株。其他血清型分离株较少。其中G、B和D型分离株SD004、SD050、SD052生长性能良好,对小鼠的LD50分别为1.99×10~(10) cfu、2.51×10~(10)cfu、5.62×10~(10)cfu。该研究为水貂出血性肺炎灭活疫苗菌株的选择提供实验依据。
[Abstract]:In order to understand the distribution of serotype of Pseudomonas aeruginosa (PA) from mink in Shandong Province, PA, was isolated from 77 samples of mink lung diseases collected from some cities and counties in Shandong Province, and identified by biochemical identification, molecular biological identification and serological identification. Growth curve and half lethal dose (LD_ _ (50) were measured. The results showed that 53 strains of PA, belonged to 11 serotypes, including 27 strains of G type B and 5 strains of D type. Other serotype isolates were less. The growth performance of SD004,SD050,SD052 was good, and the LD50 to mice was 1.99 脳 10 ~ (10) cfu,2.51 脳 10 ~ (10) cfu,5.62 脳 10 ~ (10) cfu., respectively. This study provides experimental basis for the selection of inactivated vaccine strains of mink hemorrhagic pneumonia.
【作者单位】： 东北农业大学动物医学学院;铁买克乡畜牧兽医站;
【基金】：企业横向课题“水貂出血性肺炎病原菌分离鉴定”
【分类号】：S852.61
[Abstract]:In order to understand the distribution of serotype of Pseudomonas aeruginosa (PA) from mink in Shandong Province, PA, was isolated from 77 samples of mink lung diseases collected from some cities and counties in Shandong Province, and identified by biochemical identification, molecular biological identification and serological identification. Growth curve and half lethal dose (LD_ _ (50) were measured. The results showed that 53 strains of PA, belonged to 11 serotypes, including 27 strains of G type B and 5 strains of D type. Other serotype isolates were less. The growth performance of SD004,SD050,SD052 was good, and the LD50 to mice was 1.99 脳 10 ~ (10) cfu,2.51 脳 10 ~ (10) cfu,5.62 脳 10 ~ (10) cfu., respectively. This study provides experimental basis for the selection of inactivated vaccine strains of mink hemorrhagic pneumonia.
【作者单位】： 东北农业大学动物医学学院;铁买克乡畜牧兽医站;
【基金】：企业横向课题“水貂出血性肺炎病原菌分离鉴定”
【分类号】：S852.61
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【共引文献】
相关期刊论文 前4条
1 屈腾飞;张小桐;曹Z赯，
本文编号：2239497
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