淫羊藿多糖提取工艺的优化及其对肉鸡免疫功能的调节作用
发布时间：2018-09-13 10:28
【摘要】：淫羊藿多糖(Epimedium polysaccharide)是淫羊藿(Epimedium brevicornum Maxim)的主要有效成分之一,具有调节免疫功能、抗病毒、抗氧化等多种生物活性和功能。资料表明,淫羊藿多糖是畜禽的一种良好免疫调节剂,具有较高的研究价值和应用前景。本文探索研究了淫羊藿多糖的提取工艺、含量测定及其对肉鸡生长性能和免疫调节作用的影响。1、创建一种淫羊藿多糖含量测定的方法(苯酚-硫酸法),并在此基础上,通过研究料水比、提取温度、提取时间、提取次数单因素,以及4因素3水平正交优化实验,优化淫羊藿多糖的提取工艺,并得到最佳的提取条件:料水比为1:25,提取温度为90℃,提取时间为2h,提取次数为3次。同时研究醇沉浓度,得到70%醇浓度为最佳的醇沉浓度。2、按照最佳提取条件提取淫羊藿多糖,并进行醇沉纯化,通过应用苯酚-硫酸法对淫羊藿多糖含量进行测定。测得淫羊藿粗多糖的平均得率为5.81%,淫羊藿粗多糖中多糖的平均含量是42.36%,淫羊藿中多糖平均含量为2.46%。3、将利用最佳条件提取纯化的淫羊藿多糖制成不同剂量进行动物实验,分为淫羊藿多糖实验组、免疫抑制组和空白对照组。免疫抑制组应用免疫抑制剂环磷酰胺(CY)进行免疫抑制,淫羊藿多糖实验组分别研究低剂量、中剂量、高剂量淫羊藿多糖对肉鸡的生产性能和免疫功能的调节作用。结果表明,不同剂量的淫羊藿多糖对肉鸡的平均日增重(ADG)、料肉比(FCR)等生产性能均有不同程度地促进作用;不同剂量的淫羊藿多糖对肉鸡的免疫器官指数、免疫球蛋白、细胞因子均有增强作用;在不同实验阶段,经过统计学分析,淫羊藿多糖实验组的免疫指标显著好于免疫抑制组(P0.05),并且,中、高剂量的一些免疫指标超过了空白对照组,但尚未达到差异显著(P0.05)。4、将利用最佳条件提取纯化的淫羊藿多糖制成不同剂量进行实验,分为淫羊藿多糖实验组和免疫抑制组和空白对照组。免疫抑制组应用免疫抑制剂环磷酰胺(CY)进行免疫抑制,淫羊藿多糖实验组分别研究低剂量、中剂量、高剂量淫羊藿多糖对肉鸡的肠道形态和抗氧化功能的影响。结果表明,不同剂量的淫羊藿多糖均可不同程度地增长肉鸡的肠道绒毛长度,降低隐窝深度,增加肠道内乳酸杆菌等有益菌的数量,减少肠道大肠杆菌、沙门氏菌等有害菌的数量;不同剂量的淫羊藿多糖均可不同程度地增强抗氧化物质SOD、GSH-Px、CAT的活性,降低氧化物质MDA的活性。并且,在不同实验阶段,淫羊藿多糖不同剂量组和免疫抑制组相比,上述实验指标差异显著(P0.05),而且,有些剂量的检测指标超过了空白对照组,但尚未达到差异显著(P0.05)。
[Abstract]:Epimedium polysaccharide (Epimedium polysaccharide) is one of the main active components of Epimedium (Epimedium brevicornum Maxim). The results showed that Epimedium polysaccharide was a good immunomodulator for livestock and poultry, and had high research value and application prospect. In this paper, the extraction technology of Epimedium polysaccharide, the determination of its content and its effect on growth performance and immunomodulation of broilers were studied. A method for the determination of Epimedium polysaccharide (phenol-sulfuric acid method) was established. The extraction process of Epimedium polysaccharides was optimized by orthogonal experiments of the ratio of material to water, extraction temperature, extraction time, single factor of extraction times and 4 factors and 3 levels. The optimum extraction conditions are as follows: the ratio of material to water is 1: 25, the extraction temperature is 90 鈩，
本文编号：2240913
[Abstract]:Epimedium polysaccharide (Epimedium polysaccharide) is one of the main active components of Epimedium (Epimedium brevicornum Maxim). The results showed that Epimedium polysaccharide was a good immunomodulator for livestock and poultry, and had high research value and application prospect. In this paper, the extraction technology of Epimedium polysaccharide, the determination of its content and its effect on growth performance and immunomodulation of broilers were studied. A method for the determination of Epimedium polysaccharide (phenol-sulfuric acid method) was established. The extraction process of Epimedium polysaccharides was optimized by orthogonal experiments of the ratio of material to water, extraction temperature, extraction time, single factor of extraction times and 4 factors and 3 levels. The optimum extraction conditions are as follows: the ratio of material to water is 1: 25, the extraction temperature is 90 鈩，
本文编号：2240913
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