山西省介休市猪伪狂犬病防控技术研究

发布时间：2018-09-16 20:25

【摘要】：猪伪狂犬病是由猪伪狂犬病病毒引起的猪烈性传染病,每年造成了数十亿元的直接经济损失。本文从猪伪狂犬病疫苗免疫保护率的血清学调查、疫苗免疫程序制定及病毒净化,探讨临床猪伪狂犬病防控技术,为其提供理论依据和临床实践经验。对介休地区部分规模养猪场和散养户血清样本血清学调查,规模猪场疫苗抗体平均阳性率达91%,野毒抗体平均阳性率为2.6%；散养户疫苗抗体保护率较低,平均阳性率仅为72.7%,野毒抗体平均阳性率为23.3%。规模猪场的猪伪狂犬野毒感染均不高但散养户野毒感染偏高。为制定商品猪猪伪狂犬病疫苗免疫程序,试验仔猪首免后14、21、28天,二免35天和三免35天测定血清中猪伪狂犬病病毒特异性抗体水平。对有猪伪狂犬野毒感染的猪场实施滴鼻免疫,效果理想；对保育期有感染的猪场在35日龄实施肌肉注射免疫,抗体水平较理想；对育肥期有感染的猪场,在70日龄实施肌肉注射免疫,效果较好。猪场应根据自己生产中的具体问题,合理制定免疫程序。制定“免疫—检测—淘汰—监测”循环体系,多次循环后,祖代猪伪狂犬野毒血清抗体检测均为阴性,猪伪狂犬病疫苗首免后,血清疫苗抗体显示为阳性；父母代猪血清抗体在初次免疫4周后均显示为阳性,成功完成自繁自养的猪场猪伪狂犬病净化。
[Abstract]:Porcine pseudorabies virus (PRV) is an infectious disease of swine caused by pseudorabies virus (PRV), which causes billions of yuan of direct economic losses every year. Based on the serological investigation of immune protection rate of porcine pseudorabies vaccine, the formulation of vaccine immunization procedure and virus purification, the prevention and control techniques of pseudorabies in pigs were discussed in this paper, which provided theoretical basis and clinical experience for the prevention and control of pseudorabies. The serological investigation of serum samples from some scale pig farms and scattered households in Jiexiu area showed that the average positive rate of vaccine antibody and wild virus antibody were 91% and 2.6% respectively, and the protective rate of vaccine antibody was lower in the bulk family, the average positive rate of vaccine antibody was 91%, and the average positive rate of wild virus antibody was 2.6% in Jiexiu area. The average positive rate was only 72.7 and the average positive rate of wild antibody was 23.3. The infection rate of pseudorabies wild virus in pig farm was not high, but the infection rate of wild virus was higher in bulk farm. In order to establish the immunization procedure of porcine pseudorabies vaccine, the specific antibody levels of porcine pseudorabies virus in serum were measured at 14 ~ 21g / 28 days, 35 days after first immunization and 35 days after immunization for three days after the first immunization of piglets. The effect of nasal immunization on pig farms infected with pseudorabies virus is ideal. The antibody level of infected pig farms in the care period is better than that of the pigs infected in fattening stage. At 70 days of age, the effect of intramuscular immunization is better. Pig farms should be based on their own production of specific problems, reasonable formulation of immunization procedures. The circulating system of "immuno-examination-elimination monitoring" was established. After repeated cycles, the serum antibodies of pig pseudorabies virus of ancestral generation were all negative, and the antibody of serum vaccine was positive after the first immunization of pig pseudorabies vaccine. The serum antibodies of parents on behalf of pigs were all positive after the first immunization for 4 weeks, and the purification of pseudorabies was successfully completed.
【学位授予单位】：山西农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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