猪传染性胃肠炎病毒LY86株N基因序列分析及病毒在猪睾丸细胞上的增殖特性研究
发布时间：2018-09-17 09:17
【摘要】：为探究猪传染性胃肠炎病毒(TGEV)LY86株与其他不同来源TGEV毒株之间在分子生物学上的差异及其在猪睾丸(ST)细胞上的增殖特性,分别进行了TGEV LY86株N基因的序列分析,以及病毒株以不同感染复数(MOI)感染ST细胞的毒价测定。结果显示:TGEV LY86株与其他17株TGEV参考毒株核苷酸序列同源性在97.2%~99.8%,特别是与M6、M60、TS、H、H16及JS2012毒株之间均存在很高的同源性;感染复数直接影响TGEV LY86株在ST细胞上的增殖效率,过高或过低的感染复数均可导致较低的TGEV增殖效价;当以MOI=0.001接毒时,感染后48 h所收获的病毒毒价最高,达10~(6.20±0.23)TCID_(50)/0.1 mL。本研究对分析TGEV的遗传变异规律与感染特征、优化疫苗生产工艺等具有重要的参考意义。
[Abstract]:In order to explore the molecular biological differences between (TGEV) LY86 and other TGEV strains and their proliferative characteristics in (ST) cells of swine testis, the N gene sequence of TGEV LY86 strain was sequenced. And the virulence of ST cells infected with different number of (MOI) was determined. The results showed that the nucleotide sequence homology of TGEV LY86 strain with the other 17 TGEV reference strains was 97.2% and 99.8%, especially with M6M60TGE HH16 and JS2012 strains, and the complex number of infection directly affected the proliferation efficiency of TGEV LY86 strain on ST cells. Too high or too low number of infection can lead to lower TGEV proliferation titer, when MOI=0.001 was inoculated, the virus price was the highest at 48 h after infection, reaching 10 ~ (6.20 卤0.23) TCID_ (50) / 0.1 mL.. This study is of great significance for the analysis of genetic variation and infection characteristics of TGEV and the optimization of vaccine production process.
【作者单位】： 南京农业大学动物医学院;江苏省农业科学院国家兽医疫苗工程技术研究中心;
【基金】：国家自然科学基金项目(31672515)
【分类号】：S852.651
本文编号：2245408
[Abstract]:In order to explore the molecular biological differences between (TGEV) LY86 and other TGEV strains and their proliferative characteristics in (ST) cells of swine testis, the N gene sequence of TGEV LY86 strain was sequenced. And the virulence of ST cells infected with different number of (MOI) was determined. The results showed that the nucleotide sequence homology of TGEV LY86 strain with the other 17 TGEV reference strains was 97.2% and 99.8%, especially with M6M60TGE HH16 and JS2012 strains, and the complex number of infection directly affected the proliferation efficiency of TGEV LY86 strain on ST cells. Too high or too low number of infection can lead to lower TGEV proliferation titer, when MOI=0.001 was inoculated, the virus price was the highest at 48 h after infection, reaching 10 ~ (6.20 卤0.23) TCID_ (50) / 0.1 mL.. This study is of great significance for the analysis of genetic variation and infection characteristics of TGEV and the optimization of vaccine production process.
【作者单位】： 南京农业大学动物医学院;江苏省农业科学院国家兽医疫苗工程技术研究中心;
【基金】：国家自然科学基金项目(31672515)
【分类号】：S852.651
【相似文献】
相关期刊论文 前8条
1 冉玉琴;慕晓玲;何大为;徐燕;秦波;;人骨髓间充质干细胞表型及其增殖特性[J];中国组织工程研究与临床康复;2007年15期
2 杨占秋,刘建军,肖红,文利,郭淑芳,宋婕萍;柯萨奇B组病毒在Hep-2细胞内增殖特性的研究[J];中国病毒学;1999年02期
3 何旦莎,吕明雄,Sye F. Aly,Ronald W. Hart;应用流式细胞术分析不同年龄大鼠脑组织细胞周期增殖特性[J];广东解剖学通报;1994年02期
4 金继昌;赵立红;乔健;胡永峰;田勇;赵慧颖;侯海燕;;NDV-Lasota毒株在鸡胚成纤维细胞上的增殖特性[J];实验动物科学;2007年03期
5 汪涛;马祥瑞;王洪云;;豚鼠骨髓细胞在小鼠体内的增殖特性[J];细胞生物学杂志;1980年04期
6 安靓,李进,刘小蓉;单核细胞增殖特性的实验研究[J];广东解剖学通报;1995年02期
7 丁树标;朱敏生;朱有华;虞冠华;施凤霞;;异体成人脾LAK细胞的增殖特性及其临床应用可能性研究[J];南京大学学报(自然科学版);1991年03期
8 ;[J];;年期
相关硕士学位论文 前1条
1 张培培;西达本胺对不同增殖特性弥漫大B细胞淋巴瘤体外作用机制的研究[D];郑州大学;2016年
，本文编号：2245408
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2245408.html
