ε-聚赖氨酸在牛胚胎玻璃化冷冻中的保护作用

发布时间：2018-09-18 14:51

【摘要】：通过研究ε-聚赖氨酸(ε-PL)对牛胚胎玻璃化冷冻的影响,探讨ε-PL在牛胚胎玻璃化冷冻中的保护作用,为优化牛胚胎玻璃化冷冻技术提供依据。单独以ε-PL作为冷冻保护剂,其质量浓度为20、35、50g/L,分别进行胚胎冷冻,结果均不能取得成活胚胎,说明ε-PL不能单独作为冷冻保护剂使用。在传统玻璃化冷冻剂中分别添加质量浓度为10、20、30g/L的ε-PL进行胚胎冷冻,结果发现添加ε-PL之后的冷冻保护液具有更好的保护效果,且ε-PL质量浓度为20g/L时效果最好,囊胚解冻孵化率可达42.37%。进一步探索了用该冷冻保护剂,缓冲时间分别为5、7.5、10min进行胚胎冷冻的效果,结果发现缓冲时间为7.5 min取得最高孵化率,孵化率为46.58%。结果表明,ε-PL对牛胚胎玻璃化冷冻具有一定的保护作用,且适当延长缓冲时间可取得更好的冷冻效果。
[Abstract]:By studying the effect of 蔚 -PL on vitrification of bovine embryos, the protective effect of 蔚 -PL on vitrification of bovine embryos was discussed, which provided the basis for optimizing vitrification of bovine embryos. Using 蔚 -PL alone as cryopreservation agent, the concentration of 蔚 -PL was 2055g / L, and the embryos were frozen separately. The results showed that 蔚 -PL could not be used as cryopreservation protectant alone. Embryo freezing was carried out by adding 蔚 -PL with a mass concentration of 10 ~ 20g / L or 30 g / L into the traditional vitrification refrigerant. The results showed that the cryopreservation solution with 蔚 -PL had better protective effect, and the best effect was when the concentration of 蔚 -PL was 20g/L. The hatching rate of blastocyst thawed can reach 42.37%. The effect of embryo freezing with this freezing protectant was further explored. The results showed that the highest hatching rate was obtained when the buffer time was 7.5 min, and the incubation rate was 46.58%. The results showed that 蔚 -PL had a certain protective effect on vitrification of bovine embryos, and a better freezing effect could be obtained by prolonging the buffer time properly.
【作者单位】：吉林大学动物科学学院;
【基金】：现代农业(肉牛牦牛)产业技术体系建设专项资助项目(CARS-38)
【分类号】：S823

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