蛇代谢综合征继发感染细菌的分离鉴定及致病性试验
发布时间：2018-09-19 18:16
【摘要】：近年来,广西人工饲养的蛇发生代谢障碍综合征后,病死率较高,为查清原因,对临床接诊的18例蛇代谢障碍综合征病例的心、肝、脾等脏器病料进行细菌分离鉴定,并测定分离菌对小鼠和蛇的致病力。结果从18例病蛇的不同组织器官中分离到10株细菌,PCR结合生化试验方法,鉴定出4种细菌,分别为粪肠球菌、克氏葡萄球菌、缓慢葡萄球菌和弗格森氏大肠埃希菌,并对其进行种系发育关系分析。所鉴定的4种细菌对昆明小鼠和蛇的致病力差异较大,克氏葡萄球菌和弗格森氏大肠埃希菌对昆明小鼠有较强致病性,粪肠球菌和缓慢葡萄球菌对昆明小鼠致病力较弱;克氏葡萄球菌对眼镜蛇有致病性,其余菌对王锦蛇、眼镜蛇、滑鼠蛇均无致病性。
[Abstract]:In recent years, the mortality of serpent metabolic disorder syndrome in Guangxi was high. In order to find out the cause, bacteria isolation and identification of heart, liver, spleen and other organ diseases were carried out in 18 cases of serpent metabolic disorder syndrome. The pathogenicity of isolated bacteria to mice and snakes was determined. Results 10 strains of bacteria were isolated from different tissues and organs of 18 cases of diseased snake. Four kinds of bacteria were identified by PCR and biochemical test. They were Enterococcus faecalis, Staphylococcus Crosenbergii, Staphylococcus slowus and Ferguson's Escherichia coli. The phylogenetic relationship was analyzed. The pathogenicity of the four bacteria to Kunming mice and snakes was different. Staphylococcus Crosenbergii and Ferguson's Escherichia coli had strong pathogenicity to Kunming mice, while Enterococcus faecalis and Staphylococcus slowus had weak pathogenicity to Kunming mice. Staphylococcus Crosenbergii had pathogenicity to Cobra, while the other bacteria had no pathogenicity to Snake, Cobra and Mouse Snake.
【作者单位】： 广西大学动物科学技术学院;广西兽医研究所;广西兽医生物技术重点实验室;广西陆生野生动物救护研究中心与疫源疫病监测中心;
【基金】：南宁市2016年科技项目(20163355)
【分类号】：S858.93
本文编号：2250938
[Abstract]:In recent years, the mortality of serpent metabolic disorder syndrome in Guangxi was high. In order to find out the cause, bacteria isolation and identification of heart, liver, spleen and other organ diseases were carried out in 18 cases of serpent metabolic disorder syndrome. The pathogenicity of isolated bacteria to mice and snakes was determined. Results 10 strains of bacteria were isolated from different tissues and organs of 18 cases of diseased snake. Four kinds of bacteria were identified by PCR and biochemical test. They were Enterococcus faecalis, Staphylococcus Crosenbergii, Staphylococcus slowus and Ferguson's Escherichia coli. The phylogenetic relationship was analyzed. The pathogenicity of the four bacteria to Kunming mice and snakes was different. Staphylococcus Crosenbergii and Ferguson's Escherichia coli had strong pathogenicity to Kunming mice, while Enterococcus faecalis and Staphylococcus slowus had weak pathogenicity to Kunming mice. Staphylococcus Crosenbergii had pathogenicity to Cobra, while the other bacteria had no pathogenicity to Snake, Cobra and Mouse Snake.
【作者单位】： 广西大学动物科学技术学院;广西兽医研究所;广西兽医生物技术重点实验室;广西陆生野生动物救护研究中心与疫源疫病监测中心;
【基金】：南宁市2016年科技项目(20163355)
【分类号】：S858.93
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