雌激素受体在间情期绵羊输卵管上皮细胞中的表达

发布时间：2018-10-04 21:52

【摘要】：为探讨膜性雌激素受体GPER(G protein-coupled estrogen receptor 1)及雌激素核受体ERα、ERβ在绵羊输卵管上皮细胞中的表达和定位,探讨三种受体在间情期绵羊输卵管中的表达规律,通过RT-qPCR方法和免疫印迹方法分别检测了三种受体基因mRNA水平和蛋白水平的表达差异;运用免疫组化法对输卵管组织中三种受体基因进行定位并对结果进行光密度值分析。结果,输卵管上皮细胞中GPER相对表达量高于ERα、ERβ(P0.01),ERα的相对表达量高于ERβ(P0.05)。免疫组化结果显示,GPER广泛分布于绵羊输卵管上皮细胞中,主要定位于细胞膜及细胞质。而ERα及ERβ主要分布于绵羊输卵管上皮细胞的细胞核。阳性细胞光密度分析结果与荧光定量RT-qPCR的分析结果相一致。上述结果表明,间情期绵羊输卵管上皮细胞中同时存在GPER、ERα、ERβ三种雌激素受体表达,且GPER的平均光密度、mRNA相对表达量最高,ERβ的平均光密度、mRNA相对表达量最低。
[Abstract]:To investigate the expression and localization of membrane estrogen receptor (GPER (G protein-coupled estrogen receptor 1) and estrogen nuclear receptor (ER 伪 er 尾) in ovine oviductal epithelial cells, and to investigate the expression of these receptors in ovine oviduct during interoestrous period. The expression differences of mRNA and protein levels of three receptor genes were detected by RT-qPCR and Western blotting, and the three receptor genes in oviducts were located by immunohistochemical method and the results were analyzed by optical density. The results showed that the relative expression of GPER in oviduct epithelial cells was higher than that in ER 伪 -ER 尾 (P0.01) and ER 尾 (P0.05). Immunohistochemical results showed that GPER was widely distributed in ovine oviduct epithelial cells, mainly located in cell membrane and cytoplasm. However, ER 伪 and ER 尾 mainly distributed in the nuclei of ovine oviduct epithelial cells. The results of optical density analysis of positive cells were consistent with those of fluorescent quantitative RT-qPCR. The results showed that there were three estrogen receptors of GPER,ER 伪 -ER 尾 in oviductal epithelial cells of sheep during interoestrous period, and the relative expression of GPER was the highest and the lowest was the average optical density of ER 尾.
【作者单位】：内蒙古农业大学兽医学院;内蒙古医科大学新药安全评价研究中心;
【分类号】：S826

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