4株NDV流行株的分离鉴定、生物学特性及遗传学特性的研究

发布时间：2018-10-05 07:25

【摘要】：为研究近年国内的新城疫病毒(Newcastle disease virus,NDV)流行株与传统疫苗株(Mukteswar、La Sota及Clone 30)的亲缘关系和遗传演化情况,试验从广东、广西和海南地区患病的免疫鸡群病料中分离鉴定得到4株NDV流行株。通过RT-PCR技术对各毒株的F和HN基因进行扩增、克隆与序列分析,然后与GenBank上发表的NDV序列进行同源性比对、遗传进化树分析,并测定各毒株致病指数。结果显示,本试验中HN-08株的鸡胚半数感染量(EID50)、平均死亡时间(MDT)、脑内致病指数(ICPI)和静脉致病指数(IVPI)分别为10-8.37/0.2mL、58.5h、1.78和2.45,XX-08株分别为10-6.50/0.2 mL、75.0h、1.61和2.41,YS-09株分别为10-7.75/0.2mL、64.5h、1.71和2.38,LF-09株分别为10-7.60/0.2mL、63.8h、1.84和2.38。4株鸡源NDV流行株彼此间F和HN基因推导的氨基酸序列同源性在95.8%以上,与鸡源流行株GX11/03、GM株的同源性为96.8%~98.6%,而疫苗株Mukteswar、La Sota及Clone 30株的同源性为86.7%~90.6%,与标准强毒株F48E9的同源性为88.4%~91.3%。表明4株NDV流行株均为强毒株,与近年来国内流行的GX11/03、GM株有较近的亲缘关系,而与传统疫苗株的关系相对较远。
[Abstract]:In order to study the genetic relationship and genetic evolution between Newcastle disease virus (Newcastle disease virus,NDV) epidemic strains and traditional vaccine strains (Mukteswar,La Sota and Clone 30) in recent years, the experiment was conducted from Guangdong Province. Four epidemic strains of NDV were isolated and identified from infected chickens in Guangxi and Hainan. The F and HN genes of each strain were amplified by RT-PCR, cloned and sequenced, then compared with the NDV sequence published on GenBank, analyzed the genetic evolution tree, and determined the pathogenicity index of each strain. The results show that In this study, the mean death time (MDT),) of chicken embryo half infective capacity (EID50), mean time of death (MDT), intracerebral pathogenicity index (ICPI) and venous pathogenicity index (IVPI) of HN-08 strain were 10-8.37% -0.2mLL 58.5 h 1.78 and 2.45% XX-08 strain, respectively, 10-6.50% 0.2 mL,75.0h,1.61 and 2.41% YS-09 strain were 10-7.75% 0.2mLL1.71 and 2.38 mLF-09 strain, respectively, were 10-7.600.2mLL1.84 and 2.38.4 strains of chicken-derived NDV, respectively. The deduced amino acid sequence homology of F and HN genes was more than 95.8%. The homology with GX11/03,GM strain was 96.898. 6, while the homology of Mukteswar,La Sota and Clone 30 strains was 86.7%. The homology with standard virulent strain F48E9 was 88.4% and 91.3%. The results showed that all of the four NDV strains were virulent strains, which were closely related to the GX11/03,GM strains that had been prevalent in China in recent years, but far apart from the traditional vaccine strains.
【作者单位】：佛山科学技术学院生命科学学院;
【基金】：基金项目:广东省家禽重大传染病控制技术研究(2012A020100001)
【分类号】：S852.651

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