猪圆环病毒2型诱导小鼠体内免疫细胞氧化胁迫模型的建立
发布时间：2018-10-08 19:16
【摘要】：试验旨在建立猪圆环病毒2型(PCV2)诱导的小鼠体内免疫细胞氧化胁迫模型。筛选PCV2感染剂量及感染时间,采用腹腔注射、滴鼻和灌胃3种途径联合方式于第1、2、3天或第1、3、5、7天给予昆明系小鼠感染PCV2病毒原液或10-1 PCV2病毒液,分别于感染后7、14、21d剖杀小鼠,测定活性氧(ROS)、总谷胱甘肽(T-GSH)、还原型谷胱甘肽(GSH)、氧化型谷胱甘肽(GSSG)水平及黄嘌呤氧化酶(XOD)、髓过氧化物酶(MPO)、诱导型一氧化氮合酶(iNOS)活性,探讨PCV2感染时间与活性氧水平变化的关系,建立免疫细胞氧化胁迫动物模型。结果显示,第1、2、3天每天经3种途径联合感染PCV2病毒原液,1mL/只,为后续试验感染最佳方案。PCV2感染小鼠3个时间点细胞内ROS水平较空白对照组均极显著升高(P0.01),感染后7、14dGSH水平显著降低(P0.05);感染后7dGSSG水平极显著高于空白对照组(P0.01);感染后7dT-GSH水平显著低于空白对照组(P0.05),感染后14dT-GSH水平极显著低于空白对照组(P0.01)。感染后7d脾脏XOD、MPO、iNOS活性与空白对照组相比均存在显著差异(P0.05)。结果表明,PCV2成功感染小鼠,试验感染方案为第1、2、3天每天经3种途径联合感染PCV2病毒原液,1mL/只,且用PCV2病毒原液感染7d是建立小鼠体内免疫细胞氧化胁迫模型的最佳条件。
[Abstract]:The aim of this study was to establish an oxidative stress model of immune cells induced by porcine circovirus type 2 (PCV2) in mice. The dose and time of PCV2 infection were screened, and the mice were killed by intraperitoneal injection, nasal drip and intragastric administration on the 3rd day of the first day of infection or on the 1st day of the 3rd day or the 7th day of the first day of infection with PCV2 virus or 10-1 PCV2 virus solution. The mice were killed on the 7th day, 14th day, 21d after infection, respectively. The levels of total glutathione (T-GSH), reduced glutathione (GSH), oxidized glutathione (GSSG) and (MPO), inducible nitric oxide synthase (iNOS) activity of (XOD), myeloperoxidase of xanthine oxidase were measured in order to explore the relationship between the infection time of PCV2 and the level of reactive oxygen species. The animal model of oxidative stress of immune cells was established. The results showed that 1 mL / mouse of PCV2 virus was co-infected by three ways every day on the first day of the second day. The level of intracellular ROS in PCV2 infected mice was significantly higher than that in the control group (P0.01), the level of GSH was significantly decreased at 7d after infection (P0.05), the level of 7dGSSG after infection was significantly higher than that of the control group (P0.01), and the level of 7dGSSG was significantly higher in PCV2 infected mice than in the control group (P0.01). The level of 7dT-GSH after infection was significantly lower than that of control group (P0.05), and the level of 14dT-GSH after infection was significantly lower than that of blank control group (P0.01). There were significant differences in spleen XOD,MPO,iNOS activity between the control group and the control group 7 days after infection (P0.05). The results showed that PCV2 was successfully infected in mice, and the optimal condition for the establishment of oxidative stress model of immune cells in mice was established by using PCV2 virus solution for 7 days.
【作者单位】： 广西大学动物科学技术学院;
【基金】：国家自然科学基金资助项目(31260619) 高等学校博士学科点专项科研基金博导类资助课题(20134501110004) 广西研究生教育创新计划资助项目(YCSZ2015038) 广西研究生教育创新计划项目学位与研究生教育改革专项课题(JGY2015002)
【分类号】：S852.651
[Abstract]:The aim of this study was to establish an oxidative stress model of immune cells induced by porcine circovirus type 2 (PCV2) in mice. The dose and time of PCV2 infection were screened, and the mice were killed by intraperitoneal injection, nasal drip and intragastric administration on the 3rd day of the first day of infection or on the 1st day of the 3rd day or the 7th day of the first day of infection with PCV2 virus or 10-1 PCV2 virus solution. The mice were killed on the 7th day, 14th day, 21d after infection, respectively. The levels of total glutathione (T-GSH), reduced glutathione (GSH), oxidized glutathione (GSSG) and (MPO), inducible nitric oxide synthase (iNOS) activity of (XOD), myeloperoxidase of xanthine oxidase were measured in order to explore the relationship between the infection time of PCV2 and the level of reactive oxygen species. The animal model of oxidative stress of immune cells was established. The results showed that 1 mL / mouse of PCV2 virus was co-infected by three ways every day on the first day of the second day. The level of intracellular ROS in PCV2 infected mice was significantly higher than that in the control group (P0.01), the level of GSH was significantly decreased at 7d after infection (P0.05), the level of 7dGSSG after infection was significantly higher than that of the control group (P0.01), and the level of 7dGSSG was significantly higher in PCV2 infected mice than in the control group (P0.01). The level of 7dT-GSH after infection was significantly lower than that of control group (P0.05), and the level of 14dT-GSH after infection was significantly lower than that of blank control group (P0.01). There were significant differences in spleen XOD,MPO,iNOS activity between the control group and the control group 7 days after infection (P0.05). The results showed that PCV2 was successfully infected in mice, and the optimal condition for the establishment of oxidative stress model of immune cells in mice was established by using PCV2 virus solution for 7 days.
【作者单位】： 广西大学动物科学技术学院;
【基金】：国家自然科学基金资助项目(31260619) 高等学校博士学科点专项科研基金博导类资助课题(20134501110004) 广西研究生教育创新计划资助项目(YCSZ2015038) 广西研究生教育创新计划项目学位与研究生教育改革专项课题(JGY2015002)
【分类号】：S852.651
【参考文献】
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1 林丹丹;秦韬;任U，
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