富维生素E转基因玉米对0~3周龄肉仔鸡生产性能和抗氧化能力的影响
发布时间：2018-10-09 14:58
【摘要】：试验旨在研究玉米-豆粕型饲粮中普通玉米用富维生素E转基因玉米完全替代或添加外源合成维生素E对0~3周龄AA肉仔鸡生产性能、血清α-生育酚含量、抗氧化能力和血清生化指标的影响,综合评定富维生素E转基因玉米的营养有效性,为富维生素E转基因玉米的推广应用提供数据支持。试验采用单因子随机分组试验设计,选用1日龄体质健康、体重接近的爱拔益加(AA)肉仔鸡公雏480只,随机分为3组,分别接受玉米-豆粕型饲粮(对照组1,维生素E含量为7.9mg/kg)、对照组1+7.35mg/kgdl-α-生育酚醋酸酯(对照组2,维生素E含量为15.25mg/kg)和富维生素E转基因玉米饲粮(试验组,用富维生素E转基因玉米完全替代对照组1饲粮中玉米,维生素E含量为15.25mg/kg),每组设8个重复,每个重复20只鸡,试验期为21天。试验结果表明:(1)用富维生素E转基因玉米替代玉米-豆粕型饲粮(维生素E水平为7.9mg/kg)中的普通玉米,饲粮中维生素E提高到15.25mg/kg,对0~21d肉仔鸡日增重、饲料转化率和0~7d与8~14d采食量无显著影响(P0.05),对7d与14d血清中α-生育酚含量、血清MDA含量、14d与21d血清GSH-Px活性与T-SOD活性无显著影响(P0.05),对7d与14d肝脏MDA含量、GSH-Px活性和T-SOD活性无显著影响(P0.05),对血清ALT、AST、LDH活性和BUN、TG、TC、TP、ALB、GLB和IL-2含量及A/G无显著影响(P0.05);显著降低了15~21d与全期(0~21d)采食量和21d肝脏MDA含量(P0.05);显著提高了7d血清和21d肝脏GSH-Px、T-SOD活性(P0.05)。(2)在玉米-豆粕型饲粮(维生素E水平为7.9mg/kg)中添加7.35mg/kg外源dl-α-生育酚醋酸酯,饲粮中维生素E水平提高到15.25mg/kg,对0~21 d肉仔鸡日增重、饲料转化率和采食量无显著影响(P0.05),对7d与14 d血清中α-生育酚含量、血清MDA含量与GSH-Px活性、14d与21 d血清T-SOD活性无显著影响(P0.05),对肝脏MDA含量、7d与21 d GSH-Px活性无显著影响(P0.05),对血清ALT、AST、LDH活性和BUN、TG、TC、TP、ALB、GLB和IL-2含量及A/G无显著影响(P0.05);显著提高了7d血清、21d肝脏GSH-Px活性和肝脏T-SOD活性(P0.05)。(3)在本试验条件下,富维生素E转基因玉米中维生素E的生物学效价与天然合成的dl-α-生育酚醋酸酯相当。饲粮中维生素E水平7.9mg/kg可提高0~21d肉仔鸡的抗氧化能力。
[Abstract]:The purpose of the experiment was to study the effect of vitamin E rich transgenic corn on the production performance and serum 伪 -tocopherol content of 3-week-old AA broilers in corn-soybean meal diet. The effects of antioxidant capacity and serum biochemical indexes on the nutritional effectiveness of vitamin E enriched transgenic maize were comprehensively evaluated to provide data support for the popularization and application of vitamin E rich transgenic maize. The experiment was conducted with a single factor randomized grouping design. Four hundred and eighty male broiler chicks were randomly divided into three groups, one day old, healthy and nearly weight, and four hundred and eighty male broiler chicks were divided into three groups at random. Corn-soybean meal diet (control group 1, vitamin E content 7.9mg/kg), control group 17.35 mg / kg kgdl- 伪 -tocopherol acetate (control group 2, vitamin E content 15.25mg/kg) and vitamin E rich transgenic corn diet (experimental group) were used. Vitamin E enriched transgenic corn was used to replace the control group (1), the vitamin E content was 15.25mg/kg). Each group was divided into 8 replicates, 20 chickens per replica, and the experiment period was 21 days. The results showed that: (1) Vitamin E was increased to 15.25 mg 路kg ~ (-1) in diet instead of normal corn in corn-soybean meal diet (vitamin E level: 7.9mg/kg). The feed conversion rate and feed intake at 7 days and 8 days were not significantly affected (P0.05), but the content of 伪 -tocopherol in serum of 7 days and 14 days was not significantly affected. Serum MDA content had no significant effect on serum GSH-Px activity and T-SOD activity on 14d and 21d (P0.05), but had no significant effect on hepatic MDA content (P05) and T-SOD activity (P05), but had no significant effect on serum ALT,AST,LDH activity, BUN,TG,TC,TP,ALB,GLB and IL-2 content and AP-G activity (P0.05). The activity of GSH-Px,T-SOD in serum and liver (P0.05) at 7 d and 21 d (P0.05). (2) was significantly increased by adding 7.35mg/kg exogenous dl- 伪 -tocopherol acetate to corn-soybean meal diet (vitamin E level was 7.9mg/kg). The dietary vitamin E level increased to 15.25 mg / kg, which had no significant effect on daily gain, feed conversion rate and feed intake of broilers at day 0 ~ 21 (P0.05), but on serum 伪 -tocopherol content on day 7 and day 14. Serum MDA content and GSH-Px activity had no significant effect on serum T-SOD activity at 14d and 21d (P0.05), but there was no significant effect on liver MDA content at 7d and 21d (P0.05), but had no significant effect on serum ALT,AST,LDH activity, BUN,TG,TC,TP,ALB,GLB and IL-2 content and A- / G activity (P0.05). Liver GSH-Px activity and liver T-SOD activity (P0.05). (3) were observed at 21 d after treatment. The biological titer of vitamin E in vitamin E rich transgenic maize was similar to that of natural dl- 伪 -tocopherol acetate. Dietary vitamin E level 7.9mg/kg increased the antioxidant capacity of broilers for 0 ~ 21 days.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
本文编号：2259759
[Abstract]:The purpose of the experiment was to study the effect of vitamin E rich transgenic corn on the production performance and serum 伪 -tocopherol content of 3-week-old AA broilers in corn-soybean meal diet. The effects of antioxidant capacity and serum biochemical indexes on the nutritional effectiveness of vitamin E enriched transgenic maize were comprehensively evaluated to provide data support for the popularization and application of vitamin E rich transgenic maize. The experiment was conducted with a single factor randomized grouping design. Four hundred and eighty male broiler chicks were randomly divided into three groups, one day old, healthy and nearly weight, and four hundred and eighty male broiler chicks were divided into three groups at random. Corn-soybean meal diet (control group 1, vitamin E content 7.9mg/kg), control group 17.35 mg / kg kgdl- 伪 -tocopherol acetate (control group 2, vitamin E content 15.25mg/kg) and vitamin E rich transgenic corn diet (experimental group) were used. Vitamin E enriched transgenic corn was used to replace the control group (1), the vitamin E content was 15.25mg/kg). Each group was divided into 8 replicates, 20 chickens per replica, and the experiment period was 21 days. The results showed that: (1) Vitamin E was increased to 15.25 mg 路kg ~ (-1) in diet instead of normal corn in corn-soybean meal diet (vitamin E level: 7.9mg/kg). The feed conversion rate and feed intake at 7 days and 8 days were not significantly affected (P0.05), but the content of 伪 -tocopherol in serum of 7 days and 14 days was not significantly affected. Serum MDA content had no significant effect on serum GSH-Px activity and T-SOD activity on 14d and 21d (P0.05), but had no significant effect on hepatic MDA content (P05) and T-SOD activity (P05), but had no significant effect on serum ALT,AST,LDH activity, BUN,TG,TC,TP,ALB,GLB and IL-2 content and AP-G activity (P0.05). The activity of GSH-Px,T-SOD in serum and liver (P0.05) at 7 d and 21 d (P0.05). (2) was significantly increased by adding 7.35mg/kg exogenous dl- 伪 -tocopherol acetate to corn-soybean meal diet (vitamin E level was 7.9mg/kg). The dietary vitamin E level increased to 15.25 mg / kg, which had no significant effect on daily gain, feed conversion rate and feed intake of broilers at day 0 ~ 21 (P0.05), but on serum 伪 -tocopherol content on day 7 and day 14. Serum MDA content and GSH-Px activity had no significant effect on serum T-SOD activity at 14d and 21d (P0.05), but there was no significant effect on liver MDA content at 7d and 21d (P0.05), but had no significant effect on serum ALT,AST,LDH activity, BUN,TG,TC,TP,ALB,GLB and IL-2 content and A- / G activity (P0.05). Liver GSH-Px activity and liver T-SOD activity (P0.05). (3) were observed at 21 d after treatment. The biological titer of vitamin E in vitamin E rich transgenic maize was similar to that of natural dl- 伪 -tocopherol acetate. Dietary vitamin E level 7.9mg/kg increased the antioxidant capacity of broilers for 0 ~ 21 days.
【学位授予单位】：甘肃农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
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