PADV3自然感染猪组织中病毒含量的实时荧光定量PCR检测

发布时间：2018-10-11 11:49

【摘要】：为了解猪腺病毒3型(PADV3)在自然感染猪体内不同器官组织中的分布情况,利用实时荧光定量PCR检测方法,定量检测了自然感染PADV3的仔猪、妊娠母猪以及与猪圆环病毒2型(PCV2)混合感染的仔猪不同器官组织中PADV3病毒的含量。结果,在感染的猪体多种器官组织中均可检测到PADV3,但是在各器官组织中病毒的含量有差异,其中肠黏膜、肺组织、肠系膜淋巴结、扁桃体中病毒含量较高,心、肝、脾组织中病毒含量较低;在同时混合感染PCV2的仔猪肺组织、肠系膜淋巴结、扁桃体中PADV3含量显著增加。结果表明,PADV3对猪肠黏膜、肺组织、肠系膜淋巴结、扁桃体有较强的嗜性,对心、肝、脾组织的嗜性相对较弱;PCV2混合感染可促进PADV3在仔猪体内的增殖,能增强PADV3对感染器官组织的亲嗜能力。
[Abstract]:In order to understand the distribution of porcine adenovirus type 3 (PADV3) in different organs and tissues of naturally infected pigs, real-time fluorescence quantitative PCR assay was used to quantitatively detect piglets infected with PADV3. The content of PADV3 virus in different organs of pregnant sows and piglets infected with porcine circovirus type 2 (PCV2). As a result, PADV3, could be detected in various organs and tissues of infected pigs, but there were differences in the contents of virus in various organs, including intestinal mucosa, lung tissue, mesenteric lymph nodes, tonsils, heart, liver, and so on. The content of PADV3 in lung tissue, mesenteric lymph node and tonsil of piglets mixed with PCV2 increased significantly. The results showed that PADV3 had a strong eosinophilic effect on intestinal mucosa, lung tissue, mesenteric lymph nodes and tonsils, but relatively weak on heart, liver and spleen tissues, and PCV2 mixed infection could promote the proliferation of PADV3 in piglets. It can enhance the affinity of PADV3 to infected organs and tissues.
【作者单位】：四川农业大学动物医学院;四川农业大学动物疫病与人类健康四川省重点实验室;
【基金】：四川省科技支撑计划项目(2014NZ0043) 教育部“长江学者与创新团队发展计划”项目(IRT13083) 四川省“十三五”育种攻关项目(2016NYZ0052) 国家“十二五”科技支撑计划(2015BAD12B04-2.3)
【分类号】：S858.28

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