含1,2-丙二醇青贮玉米微生物菌群分析

发布时间：2018-10-11 12:09

【摘要】：为了研究袋式青贮玉米与窖式青贮玉米微生物群集的差异,同时分析与发酵产物中1,2-丙二醇相关的微生物,试验采用全株玉米进行袋式青贮以及窖式青贮。袋式青贮在3,7,14,28,56,120天开封,采样;窖式青贮选择3个500 t级别的半地上式青贮窖,贮藏120 d后每个青贮窖选择8个采样点进行采样。使用聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)方法检测微生物区系并进行聚类分析。结果表明:袋式青贮玉米发酵过程中微生物区系无变化,在其中检测到类肠膜魏斯氏菌(W.paramesenteroides)、魏斯氏菌(W.confusa)、肺炎克雷伯菌(K.pneumoniae)、植物乳杆菌(L.plantarum)、短乳杆菌(L.brevis)和乳酸乳杆菌(L.lactis)。青贮窖不同位置微生物菌群具有一定差异,耐酸乳杆菌(L.acetotolerans)、植物乳杆菌(L.plantarum)和布氏乳杆菌(L.buchneri)为3个青贮窖共通菌,同时还检测到了屎肠球菌(E.faecium)、巴氏醋杆菌(A.pasteurianus)、罗伊氏乳杆菌(L.reuteri)、面包乳杆菌(L.panis)、嗜麦芽窄食单胞菌(S.maltophilia)和类肠膜魏斯氏菌(W.Paramesenteroides)。说明袋式青贮微生物菌群与窖式青贮微生物菌群具有一定差异,窖式青贮中检测到了一些在袋式青贮中不常见的乳酸菌;青贮窖中1,2-丙二醇的生成可能与耐酸乳杆菌(L.acetotolerans)、巴氏醋杆菌(A.pasteurianus)、罗伊氏乳杆菌(L.reuteri)、面包乳杆菌(L.panis)和布氏乳杆菌(L.buchneri)有关。
[Abstract]:In order to study the difference of microbial colony between corn with bag silage and cellar silage, and to analyze the microorganism related to 1o 2-propanediol in fermentation product, the whole plant corn was used to carry out bag silage and cellar silage. Three half-ground silages of 500t grade were selected for silage, and 8 sampling sites were selected for each silage after 120 days of storage. Microbial flora was detected by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and cluster analysis was carried out. The results showed that there was no change in microbial flora during the fermentation of silage corn. Among them, W.paramesenteroides, W.confusa, K.pneumoniae, L.plantarum, L.brevis and L.lactis were detected. The microbial flora of silage cellar was different in different locations. Lactobacillus acidophilus (L.acetotolerans), Lactobacillus plantarum (L.plantarum) and Lactobacillus brucelis (L.buchneri) were three common bacteria in silage cellar. E.faecium, A.pasteurianus, L.reuteri, L.panis, S.maltophilia and W.Paramesenteroides were also detected. The results showed that there were some differences between the microbial flora of bag silage and that of cellar silage, and some rare lactic acid bacteria were detected in the cellar silage. The production of 1 ~ 2-propanediol in silage cellar may be related to Lactobacillus acidophilus (L.acetotolerans), Acetobacter pasteurus (A.pasteurianus), Lactobacillus Roy (L.reuteri), Lactobacillus bread (L.panis) and Lactobacillus bruceli (L.buchneri).
【作者单位】：黑龙江八一农垦大学动物科技学院;东北农业大学动物科学技术学院;云南农业大学动物科学技术学院;
【基金】：国家自然科学基金项目(31402136) 黑龙江省应用技术研究与开发计划项目(WB13B111) 黑龙江省博士后基金项目(LBH-Z14029) 黑龙江省垦区科研项目(HNK135-04-02-03) 黑龙江八一农垦大学博士启动资金项目(B2012-06)
【分类号】：S816.53

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