济宁青山羊出生后胃肠道中GnRH及GnRHR的发育性变化
发布时间：2018-10-12 11:34
【摘要】：原产自我国的济宁青山羊是一种独特的羔皮用山羊品种,其特性是四季发情,性成熟早,母羊在3月龄、公羊在4月龄发育至性成熟。济宁青山羊的初情期开始于3-4月龄,部分发育较早的个体于2月龄开始初情期,其发情周期为17.50d±0.50d,发情持续时间为1-2d,妊娠期约为147.00d±2.50 d,平均146d。济宁青山羊的繁殖率高,其羔羊的成活率高达95%。其性情温驯易于管理,遗传性稳定,适应能力强,耐粗饲,具有很高的经济价值,现已被列入《中国羊品种志》,属于优良的地方山羊品种。研究济宁青山羊胃肠道促性腺激素释放激素(GnRH)及促性腺激素释放激素受体(GnRHR)分布的增龄性变化对于了解GnRH及GnRHR对动物生后发育阶段消化道的功能产生的影响提供了必要的理论依据。为了解济宁青山羊生后发育时期胃肠道内GnRH和GnRHR的分布和发育性变化。本试验运用免疫组织化学(SABC)的方法检测了济宁青山羊出生当天(0日龄)、30日龄、60日龄、90日龄、120日龄、150日龄、180日龄胃肠道中GnRH和GnRHR免疫阳性细胞的动态分布。在胃肠道内,GnRH和GnRHR免疫阳性产物均定位在胞质中,为黄色或者棕黄色,胞核阴性,阴性对照无阳性着色。0日龄,GnRH免疫阳性产物在皱胃中分布在胃底腺的基底部、小肠的绒毛上皮之间、大肠的大肠腺杯状细胞之间;30日龄后,小肠腺上皮细胞中也出现了分布,其他各部位的免疫阳性物质明显增多,并且保持不断的增长趋势,皱胃中GnRH免疫阳性产物在60日龄达到峰值,其他部位在90日龄达到峰值。在济宁青山羊的0日龄时,GnRHR免疫阳性产物在胃底腺胃小凹上皮细胞与壁细胞中、小肠的绒毛上皮细胞与十二指肠腺上皮细胞中、大肠黏膜上皮细胞中;30日龄后,大肠腺的腺上皮细胞中也出现了分布,随后保持快速增长的趋势直至90日龄达到峰值。自0日龄-90日龄,GnRH和GnRHR免疫阳性细胞平均积分光密度增加均极显著(P0.01),90日龄后维持相对稳定(P0.05)。综上所述,笔者推测济宁青山羊胃肠道还可以通过自分泌或旁分泌的形式合成与分泌GnRH;GnRH可能通过GnRHR的介导促进了胃肠道的生后发育及成熟过程。
[Abstract]:Jining Grey Goat (Jining), which originated from China, is a unique goat breed for lamb skin, which is characterized by four seasons of estrus, early sexual maturity, 3 months old ewe and 4 months old male sheep. The initial estrous period of Jining green goat begins at 3-4 months old, and some of the early developing individuals begin their early estrous period at the age of 2 months. The estrus cycle is 17.50 days 卤0.50 days, the estrus duration is 1-2 days, and the gestation period is about 147.00 卤2.50 days, with an average of 146 days. The reproduction rate of Jining green goat is high, and the survival rate of lamb is up to 95%. Its temperament is easy to manage, genetic stability, strong adaptability, tolerance to coarse feeding, has a high economic value, has been listed in the "Chinese Sheep Variety", belong to the excellent local goat breeds. Study on the Aging changes of Gastrointestinal Gonadotropin releasing hormone (GnRH) and Gonadotropin releasing hormone receptor (GnRHR) Distribution in Jining Green Goat provides a way to understand the effects of GnRH and GnRHR on the gastrointestinal function during postnatal development of animals The necessary theoretical basis is given. To investigate the distribution and developmental changes of GnRH and GnRHR in gastrointestinal tract of Jining green goat during postnatal development. The dynamic distribution of GnRH and GnRHR immunoreactive cells in the gastrointestinal tract of Jining green goats on the day of birth (0 days old), 30 days old, 60 days old, 90 days old, 120 days old, 150 days old and 180 days old was detected by immunohistochemical (SABC) method. In the gastrointestinal tract, GnRH and GnRHR immunoreactive products were located in the cytoplasm, yellow or brown, negative in nucleus, and no positive staining in negative control. At 0 days of age, GnRH immunoreactive products were distributed in the basal base of fundus gland in the abomasum. Between villous epithelium of the small intestine and goblet cells of the large intestine, after 30 days of age, the epithelial cells of the small intestine also appeared to be distributed, and the immunoreactive substances in the other parts of the intestine increased obviously and kept the increasing trend. The GnRH immunoreactive products in the abomasum reached the peak at 60 days old and the other parts at 90 days old. GnRHR immunoreactive products were found in gastric fundus gland gastric fovea epithelial cells and parietal cells, small intestinal villus epithelial cells and duodenal gland epithelial cells, large intestine mucosal epithelial cells, and 30 days after 30 days of age. The distribution of glandular epithelial cells in the large intestine gland also appeared, and then maintained a rapid growth trend until 90 days of age reached a peak. From day 0 to day 90, the mean integral optical density of GnRH and GnRHR immunoreactive cells increased significantly (P0.01), and remained relatively stable after 90 days of age (P0.05). To sum up, we speculated that the gastrointestinal tract of Jining green goat can also synthesize and secrete GnRH;GnRH through autocrine or paracrine, which may promote the development and maturation of gastrointestinal tract through the mediation of GnRHR.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S827
本文编号：2265953
[Abstract]:Jining Grey Goat (Jining), which originated from China, is a unique goat breed for lamb skin, which is characterized by four seasons of estrus, early sexual maturity, 3 months old ewe and 4 months old male sheep. The initial estrous period of Jining green goat begins at 3-4 months old, and some of the early developing individuals begin their early estrous period at the age of 2 months. The estrus cycle is 17.50 days 卤0.50 days, the estrus duration is 1-2 days, and the gestation period is about 147.00 卤2.50 days, with an average of 146 days. The reproduction rate of Jining green goat is high, and the survival rate of lamb is up to 95%. Its temperament is easy to manage, genetic stability, strong adaptability, tolerance to coarse feeding, has a high economic value, has been listed in the "Chinese Sheep Variety", belong to the excellent local goat breeds. Study on the Aging changes of Gastrointestinal Gonadotropin releasing hormone (GnRH) and Gonadotropin releasing hormone receptor (GnRHR) Distribution in Jining Green Goat provides a way to understand the effects of GnRH and GnRHR on the gastrointestinal function during postnatal development of animals The necessary theoretical basis is given. To investigate the distribution and developmental changes of GnRH and GnRHR in gastrointestinal tract of Jining green goat during postnatal development. The dynamic distribution of GnRH and GnRHR immunoreactive cells in the gastrointestinal tract of Jining green goats on the day of birth (0 days old), 30 days old, 60 days old, 90 days old, 120 days old, 150 days old and 180 days old was detected by immunohistochemical (SABC) method. In the gastrointestinal tract, GnRH and GnRHR immunoreactive products were located in the cytoplasm, yellow or brown, negative in nucleus, and no positive staining in negative control. At 0 days of age, GnRH immunoreactive products were distributed in the basal base of fundus gland in the abomasum. Between villous epithelium of the small intestine and goblet cells of the large intestine, after 30 days of age, the epithelial cells of the small intestine also appeared to be distributed, and the immunoreactive substances in the other parts of the intestine increased obviously and kept the increasing trend. The GnRH immunoreactive products in the abomasum reached the peak at 60 days old and the other parts at 90 days old. GnRHR immunoreactive products were found in gastric fundus gland gastric fovea epithelial cells and parietal cells, small intestinal villus epithelial cells and duodenal gland epithelial cells, large intestine mucosal epithelial cells, and 30 days after 30 days of age. The distribution of glandular epithelial cells in the large intestine gland also appeared, and then maintained a rapid growth trend until 90 days of age reached a peak. From day 0 to day 90, the mean integral optical density of GnRH and GnRHR immunoreactive cells increased significantly (P0.01), and remained relatively stable after 90 days of age (P0.05). To sum up, we speculated that the gastrointestinal tract of Jining green goat can also synthesize and secrete GnRH;GnRH through autocrine or paracrine, which may promote the development and maturation of gastrointestinal tract through the mediation of GnRHR.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S827
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