幼龄羔羊超数排卵及体外生产胚胎技术的研究
发布时间：2018-10-12 20:29
【摘要】：东北细毛羊为肉毛兼用优良品种,本试验研究了东北细毛羊幼母羔超数排卵、卵母细胞成熟培养及体外受精的影响因素,旨在为该技术在生产实际中应用提供相关借鉴。试验共分为三部分:分别研究了羔羊超数排卵效果影响因素、卵母细胞成熟液成熟效果和精子获能方法及受精卵发育的影响因素,结果如下:1.6周龄羔羊回收卵枚数、回收率及可用卵枚数高于4周龄组和8周龄组,但差异不显著(P0.05),可用卵率显著高于4周龄组(P0.05);采用FSH+PMSG(加拿大产)超排后回收率及可用卵率均高于其它三组,其中可用卵率极显著高于FSH+LH(国产)组(P0.01);秋季可用卵率高于春季并极显著高于夏季(P0.01);首次超排卵母细胞回收率极显著高于重复超排组(P0.01),可用卵率高于重复组但无显著差异;非运输组可用卵率极显著高于运输组(P0.01),卵回收率无明显差异(P0.05)。2.比较了五种培养液的成熟效果,各组间成熟率差异不显著(P0.05),表明可以使用10mg/ml BSA替代血清(10%FBS)的添加。3.相同获能时间内,对照组获能率显著低于肝素添加组(P0.05),肝素添加组获能2h获能率达到峰值,并显著高于对照组(P0.05);25μg/mL组精子存活时间显著低于对照组(P0.05),同时显著高于50μg/mL、100μg/mL添加组(P0.05);综合结果表明,肝素浓度25μg/ml,处理2h的精子获能效果最好。卵母细胞直径130μm组第二极体率显著高于d120μm组,卵裂率显著大于120μmd130μm组(P0.05)并极显著高于d120μm组(P0.01)。卵母细胞体外成熟培养24~27h第二极体率和卵裂率高于培养27~30h,极显著高于培养21~24h(P0.01)。成熟液中添加EGF能够显著提高成熟率率和卵裂率(P0.05),极显著提高囊胚率(P0.01),添加量为20 ng/mL较10 ng/mL囊胚率有极显著提高(P0.01)。SOF体系卵裂率显著高于TALP体系(P0.05),而SOF体系囊胚率极显著高于TALP体系(P0.01)。
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【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826
本文编号：2267498
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【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S826
【参考文献】
相关硕士学位论文 前1条
1 武建朝;利用性成熟前山羊卵泡卵母细胞体外生产胚胎的研究[D];南京农业大学;2007年
，本文编号：2267498
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