益生菌增加IPEC-J2细胞表面半乳糖在抑制RV感染中的作用
发布时间：2018-10-15 12:01
【摘要】：轮状病毒通过识别细胞表面糖链附着于肠上皮细胞,一些肠道内常驻菌群可以修改细胞表面糖链。以3种益生菌上清孵育猪小肠上皮细胞IPEC-J2,猪轮状病毒(PRV)感染细胞。采用凝集素荧光技术测定各处理组IPEC-J2细胞表面半乳糖,检测对应轮状病毒滴度和RV含量变化。结果表明,对照组PRV TCID_(50)·0.1 mL~(-1)为10~(-7.15±0.14),干酪乳杆菌组TCID_(50)·0.1mL~(-1)为10~(-3.875±0.125),食淀粉乳杆菌PRV TCID_(50)·0.1 mL~(-1)为10~(-4.125±0.138),枯草芽孢杆菌PRV TCID_(50)·0.1 mL~(-1)为10~(-4.16±0.144)。3组益生菌上清组在RV感染48 h内,RV量显著低于未处理组。凝集素荧光标记表明,3株益生菌上清均可改变细胞表面半乳糖含量。干酪乳杆菌、食淀粉乳杆菌和枯草芽孢杆菌上清可增加IPEC-J2细胞表面半乳糖含量,提高细胞抗RV感染能力,阻断RV黏附,保护肠道上皮细胞。
[Abstract]:Rotavirus adheres to intestinal epithelial cells by recognizing the sugar chains on the surface of the cells, and some intestinal resident bacteria can modify the sugar chains on the surface of the cells. Porcine intestinal epithelial cells (IPEC-J2,) infected with porcine rotavirus (PRV) were incubated with three probiotic supernatants. Galactose (galactose) on the surface of IPEC-J2 cells was measured by lectin fluorescence technique and the changes of rotavirus titer and RV content were detected. 缁撴灉琛ㄦ槑,瀵圭収缁凱RV TCID_(50)路0.1 mL~(-1)涓，
本文编号：2272490
[Abstract]:Rotavirus adheres to intestinal epithelial cells by recognizing the sugar chains on the surface of the cells, and some intestinal resident bacteria can modify the sugar chains on the surface of the cells. Porcine intestinal epithelial cells (IPEC-J2,) infected with porcine rotavirus (PRV) were incubated with three probiotic supernatants. Galactose (galactose) on the surface of IPEC-J2 cells was measured by lectin fluorescence technique and the changes of rotavirus titer and RV content were detected. 缁撴灉琛ㄦ槑,瀵圭収缁凱RV TCID_(50)路0.1 mL~(-1)涓，
本文编号：2272490
本文链接：https://www.wllwen.com/yixuelunwen/dongwuyixue/2272490.html
