猪IGFBP2基因遗传多态性及其与肉质性状的关联分析

发布时间：2018-10-17 11:14

【摘要】：试验旨在探究胰岛素样生长因子结合蛋白2(insulin-like growth factor binding protein 2,IGFBP2)基因遗传变异对猪肉质性状的影响,为猪品种选育提供遗传学依据。参考Gen Bank中猪IGFBP2基因序列(登录号:BV727778)设计1对引物,采用PCR-RFLP技术检测IGFBP2基因在苏姜猪、姜曲海猪、长白猪中的遗传多态性,并分析基因座多态性与猪肉质性状的相关性。结果发现,3个猪种IGFBP2基因内含子3均具有MspⅠ酶切多态性,分别检测到3种基因型:AA、AB和BB;χ2适合性检验显示,3个猪种IGFBP2基因基因型分布均处于HardyWeinberg平衡状态(P0.05);多态信息含量(PIC)分析显示,3个猪种均为中度多态。IGFBP2基因不同基因型与肉质性状关联分析显示,3个猪种不同基因型肉色、肌内脂肪含量、大理石纹差异显著,AA基因型肉色(姜曲海猪除外)、肌内脂肪含量均显著高于AB、BB基因型(P0.05);AA、AB基因型大理石纹显著高于BB基因型(P0.05),其余指标间差异不显著(P0.05)。综上,苏姜猪、姜曲海猪、长白猪IGFBP2基因内含子3多态性对肉质性状影响显著,可作为潜在影响猪肉质性状的候选基因。
[Abstract]:The aim of the experiment was to investigate the effect of genetic variation of insulin-like growth factor binding protein 2 (insulin-like growth factor binding protein _ 2 IGFBP2) gene on pork quality traits, and to provide genetic basis for pig breeding. A pair of primers were designed with reference to pig IGFBP2 gene sequence (accession number: BV727778) in Gen Bank. The genetic polymorphism of IGFBP2 gene in Sujiang pig, Jiangqu Hai pig and Landrace pig was detected by PCR-RFLP technique, and the correlation between locus polymorphism and pig meat quality traits was analyzed. The results showed that intron 3 of IGFBP2 gene of all three pig breeds had Msp 鈪，

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