牛磺酸对大鼠铅中毒的预防作用
发布时间：2018-10-19 08:24
【摘要】：铅是应用最广泛的重金属之一,长期处于铅暴露环境是引起铅中毒的主要原因,临床上铅中毒多见于职业病。进入机体后,铅会沉积在全身多个部位,对多个组织器官造成损害。牛磺酸是一种具有多项调节功能的内源性抗损伤物质,在动物体内广泛存在,本研究以Wistar大鼠为研究对象,将大鼠分为空白对照组、染铅组、牛磺酸组和牛磺酸预防组等若干组,进行不同处理后,从以下四个部分开展研究工作:1.牛磺酸对染铅大鼠生长发育的影响将大鼠分组后进行不同处理,每周记录各组大鼠的体重数据,于实验末进行大鼠腹主动脉采血制备血浆,血样消解后检测血铅含量;将心、肝、脾、肺、肾和睾丸等器官分离摘取出来,测定各组大鼠脏器系数。结果发现各组大鼠体重变化无显著差异(P0.05),铅对大鼠脾、肺、肾和睾丸的脏器系数有显著影响(P0.05),牛磺酸预防对染铅大鼠肾和肺的发育具有明显的改善作用(P0.05);染铅组大鼠血铅含量明显高于空白对照组(P0.05),且与染铅组相比,牛磺酸预防组大鼠血铅浓度明显下降(P0.05)。2.牛磺酸对染铅大鼠肝、肾、睾丸结构损伤的改善作用铅进入动物机体后很难被机体排出而在各组织器官处蓄积下来,对机体的组织、器官造成损害。本部分研究主要通过制备石蜡切片和透射电镜超薄切片,从病理组织学变化来探讨牛磺酸对铅中毒的预防作用。光学显微镜和透射电镜观察发现染铅组大鼠肝、肾、睾丸组织都发生了不同程度的损伤;与染铅组相比,牛磺酸预防组大鼠组织损伤有所改善,证实了牛磺酸对铅中毒引起的大鼠组织损伤具有预防作用。3.牛磺酸对染铅大鼠氧化损伤的预防作用铅主要通过氧化应激机制,引起机体过氧化过程,生成活性氧自由基(ROS),对机体的组织器官造成损害。本部分研究主要从测定组织和血液抗氧化生化指标来探讨牛磺酸对铅中毒的预防作用。将分离出来的大鼠组织和采集的血液分别制备处理为组织匀浆和血浆,样品前处理完成后进行组织匀浆和血浆中GSH、GSH-Px、MDA、SOD的检测。结果发现染铅组大鼠各组织和血浆中GSH、GSH-Px、SOD浓度均最低,补充牛磺酸后浓度有所升高,但仍低于空白对照组和牛磺酸组Ⅰ、牛磺酸组Ⅱ;染铅组大鼠MDA含量均最高,补充牛磺酸后浓度有所下降,但仍高于空白对照组和牛磺酸组Ⅰ、牛磺酸组Ⅱ,说明牛磺酸预防组大鼠的各项指标在一定程度上得到了改善。4.牛磺酸对染铅大鼠肝组织细胞凋亡的影响本部分研究应用TUNEL法检测各组大鼠肝细胞凋亡情况,探讨牛磺酸预防对染铅大鼠肝细胞凋亡的改善作用。结果显示,铅中毒会导致大鼠肝细胞凋亡,而经过牛磺酸预防后,细胞凋亡情况有所好转。以上结果表明,牛磺酸对大鼠铅中毒有一定的预防作用。
[Abstract]:Lead is one of the most widely used heavy metals. Exposure to lead for a long time is the main cause of lead poisoning. After entering the body, lead deposits in multiple parts of the body, causing damage to multiple tissues and organs. Taurine (taurine) is an endogenous anti-injury substance with multiple regulatory functions, which is widely present in animals. In this study, Wistar rats were divided into blank control group and lead exposed group. Taurine group and taurine prevention group and other groups, after different treatment, from the following four parts of the research work: 1. Effects of taurine on the growth and Development of lead exposed Rats the rats were divided into groups, and the body weight data of each group were recorded weekly. At the end of the experiment, blood samples were collected from the abdominal aorta of rats to prepare plasma, and blood samples were digested to detect the content of blood lead. Liver, spleen, lung, kidney and testis were isolated and the visceral coefficients were measured. The results showed that there was no significant difference in body weight change in each group (P0.05). The visceral coefficients of kidney and testis were significantly affected (P0.05), and taurine could significantly improve the development of kidney and lung of lead-exposed rats (P0.05), and the blood lead content of lead-exposed rats was significantly higher than that of blank control group (P0.05), and compared with lead-exposed rats, taurine had a significant effect on the development of kidney and lung in lead-exposed rats (P0.05). The concentration of blood lead in taurine prevention group decreased significantly (P0.05). Effects of taurine on the damage of liver, kidney and testis in lead exposed rats; lead is difficult to be excreted by the body and accumulates in various tissues and organs, which causes damage to the tissues and organs of the body. In this part, the preventive effect of taurine on lead poisoning was studied by preparing paraffin sections and ultrathin sections of transmission electron microscope. The results of optical microscope and transmission electron microscope showed that the liver, kidney and testicular tissues of the lead exposed rats were damaged to varying degrees, and that of the taurine pretreated group was better than that of the lead exposed group. It is proved that taurine has preventive effect on tissue injury induced by lead poisoning in rats. Prevention of oxidative damage induced by taurine in lead exposed rats the oxidative stress mechanism led to the formation of reactive oxygen free radical (ROS),) which caused damage to tissues and organs of the body through the mechanism of oxidative stress. In this part, the preventive effect of taurine on lead poisoning was studied by measuring the biochemical indexes of tissue and blood antioxidant. The isolated rat tissues and blood samples were prepared and treated into tissue homogenate and plasma respectively. GSH,GSH-Px,MDA,SOD in tissue homogenate and plasma were detected after sample pretreatment. The results showed that the concentration of GSH,GSH-Px,SOD was the lowest in all tissues and plasma of lead-exposed rats, and increased after taurine supplementation, but still lower than that of control group, taurine group 鈪，
本文编号：2280621
[Abstract]:Lead is one of the most widely used heavy metals. Exposure to lead for a long time is the main cause of lead poisoning. After entering the body, lead deposits in multiple parts of the body, causing damage to multiple tissues and organs. Taurine (taurine) is an endogenous anti-injury substance with multiple regulatory functions, which is widely present in animals. In this study, Wistar rats were divided into blank control group and lead exposed group. Taurine group and taurine prevention group and other groups, after different treatment, from the following four parts of the research work: 1. Effects of taurine on the growth and Development of lead exposed Rats the rats were divided into groups, and the body weight data of each group were recorded weekly. At the end of the experiment, blood samples were collected from the abdominal aorta of rats to prepare plasma, and blood samples were digested to detect the content of blood lead. Liver, spleen, lung, kidney and testis were isolated and the visceral coefficients were measured. The results showed that there was no significant difference in body weight change in each group (P0.05). The visceral coefficients of kidney and testis were significantly affected (P0.05), and taurine could significantly improve the development of kidney and lung of lead-exposed rats (P0.05), and the blood lead content of lead-exposed rats was significantly higher than that of blank control group (P0.05), and compared with lead-exposed rats, taurine had a significant effect on the development of kidney and lung in lead-exposed rats (P0.05). The concentration of blood lead in taurine prevention group decreased significantly (P0.05). Effects of taurine on the damage of liver, kidney and testis in lead exposed rats; lead is difficult to be excreted by the body and accumulates in various tissues and organs, which causes damage to the tissues and organs of the body. In this part, the preventive effect of taurine on lead poisoning was studied by preparing paraffin sections and ultrathin sections of transmission electron microscope. The results of optical microscope and transmission electron microscope showed that the liver, kidney and testicular tissues of the lead exposed rats were damaged to varying degrees, and that of the taurine pretreated group was better than that of the lead exposed group. It is proved that taurine has preventive effect on tissue injury induced by lead poisoning in rats. Prevention of oxidative damage induced by taurine in lead exposed rats the oxidative stress mechanism led to the formation of reactive oxygen free radical (ROS),) which caused damage to tissues and organs of the body through the mechanism of oxidative stress. In this part, the preventive effect of taurine on lead poisoning was studied by measuring the biochemical indexes of tissue and blood antioxidant. The isolated rat tissues and blood samples were prepared and treated into tissue homogenate and plasma respectively. GSH,GSH-Px,MDA,SOD in tissue homogenate and plasma were detected after sample pretreatment. The results showed that the concentration of GSH,GSH-Px,SOD was the lowest in all tissues and plasma of lead-exposed rats, and increased after taurine supplementation, but still lower than that of control group, taurine group 鈪，
本文编号：2280621
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