水盐胁迫下双峰驼肾脏与肠道组织中钙调素的表达
发布时间：2018-10-19 14:24
【摘要】：骆驼具有耐盐和耐干旱等显著特点。研究发现,骆驼耐盐耐旱基因的启动,可能需要钙离子的参与。钙调素通过与钙离子的结合而激活,因此,可以通过钙调素的表达来观察钙离子的变化。本实验通过给骆驼禁盐、灌盐、禁水和禁水后暴饮等处理,采取免疫荧光染色技术,对不同处理后骆驼肾皮质、肾髓质、十二指肠、回肠、空肠和结肠组织中钙调素的表达进行了研究,以探讨钙离子与骆驼水盐代谢相关基因表达的关系。其主要研究结果如下：1、克隆获得了双峰驼的钙调素基因序列,并证明其能在Hela真核细胞内表达。2、经不同处理后,双峰驼肾皮质、肾髓质、十二指肠、空肠、回肠、结肠组织中钙调素均有表达。但在不同的处理组中,其表达的强度有所不同,即在灌盐组与禁水组中较高,对照组与禁水后暴饮组次之,禁盐组中较低。3、在不同处理组的不同组织中,钙调素表达的位置分布也不同,即在肾皮质中,钙调素在肾小体足细胞与肾小管近曲小管中表达强度较高,肾小管远曲小管中较低；在肾髓质中,钙调素在肾小管的远曲小管中表达强度较高,而在集合小管中较低。在肠道组织中,钙调素在十二指肠、空肠和回肠的绒毛上皮细胞中表达强度较高,而在结肠的肠腺细胞中表达强度较高。4、不同处理不同组织中,钙调素表达的变化说明了钙离子的变化,间接说明了钙离子与骆驼水盐代谢相关基因表达的可能关系。
[Abstract]:Camel has the characteristic of salt tolerance and drought tolerance. It has been found that the initiation of salt-tolerant drought-tolerant genes in camels may require the participation of calcium ions. Calmodulin is activated by binding with calcium ion, so the change of calcium ion can be observed by the expression of calmodulin. In this experiment, the camel renal cortex, medulla, duodenum and ileum were treated with immunofluorescence staining by salt, water and drinking. The expression of calmodulin in jejunum and colon was studied in order to explore the relationship between calcium ion and the expression of genes related to water salt metabolism in camels. The main results are as follows: 1. The calmodulin gene sequence of Bactrian camel was cloned and proved to be expressed in Hela eukaryotic cells. After different treatments, the renal cortex, medulla, duodenum, jejunum, ileum of bactrian camel were obtained. Calmodulin was expressed in colon tissue. However, the intensity of expression was different in different treatment groups, that is, higher in salt irrigation group and water deprivation group, second in control group and drinking after water ban group, and lower in salt ban group, and in different tissues of different treatment groups. The expression of calmodulin in renal cortex was higher in podocytes and proximal tubules, lower in distal tubules, and in renal medulla. Calmodulin expression was higher in the distal tubules of the renal tubules than in the collective tubules. In intestinal tissues, calmodulin expression was higher in chorionic epithelial cells of duodenum, jejunum and ileum, but higher in intestinal gland cells of colon. The change of calmodulin expression indicated the change of calcium ion and indirectly explained the possible relationship between calcium ion and the expression of genes related to water salt metabolism in camels.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S824
本文编号：2281401
[Abstract]:Camel has the characteristic of salt tolerance and drought tolerance. It has been found that the initiation of salt-tolerant drought-tolerant genes in camels may require the participation of calcium ions. Calmodulin is activated by binding with calcium ion, so the change of calcium ion can be observed by the expression of calmodulin. In this experiment, the camel renal cortex, medulla, duodenum and ileum were treated with immunofluorescence staining by salt, water and drinking. The expression of calmodulin in jejunum and colon was studied in order to explore the relationship between calcium ion and the expression of genes related to water salt metabolism in camels. The main results are as follows: 1. The calmodulin gene sequence of Bactrian camel was cloned and proved to be expressed in Hela eukaryotic cells. After different treatments, the renal cortex, medulla, duodenum, jejunum, ileum of bactrian camel were obtained. Calmodulin was expressed in colon tissue. However, the intensity of expression was different in different treatment groups, that is, higher in salt irrigation group and water deprivation group, second in control group and drinking after water ban group, and lower in salt ban group, and in different tissues of different treatment groups. The expression of calmodulin in renal cortex was higher in podocytes and proximal tubules, lower in distal tubules, and in renal medulla. Calmodulin expression was higher in the distal tubules of the renal tubules than in the collective tubules. In intestinal tissues, calmodulin expression was higher in chorionic epithelial cells of duodenum, jejunum and ileum, but higher in intestinal gland cells of colon. The change of calmodulin expression indicated the change of calcium ion and indirectly explained the possible relationship between calcium ion and the expression of genes related to water salt metabolism in camels.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S824
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