新疆巴什拜羊肌肉生长抑制素基因DNA甲基化与mRNA表达量分析

发布时间：2018-10-19 19:37

【摘要】：为了探究肌肉生长抑制素(myostatin,MSTN)基因在肌肉和脂肪中的DNA甲基化模式及mRNA表达水平,试验以5月龄巴什拜羊羔羊为研究对象,采用亚硫酸氢盐测序法(bisulfite sequencing PCR,BSP)检测MSTN基因启动子区和第1外显子甲基化模式,并通过实时荧光定量PCR检测MSTN基因在巴什拜羊股二头肌、股三头肌、半腱肌、半膜肌、背最长肌和尾脂中的mRNA表达水平。结果显示,肌肉组织甲基化概率高于脂肪组织,其中,股二头肌、股三头肌、半腱肌、半膜肌、背最长肌和尾脂的甲基化概率分别为74.2%、74.2%、83.2%、83.7%、82.1%和25.3%,MSTN基因在股二头肌、股三头肌、半腱肌、半膜肌、背最长肌中的表达水平显著低于尾脂(P0.05),股二头肌、股三头肌、半腱肌、半膜肌和背最长肌之间差异不显著(P0.05),巴什拜羊肌肉和脂肪MSTN基因DNA甲基化水平与MSTN基因表达量呈显著负相关(r=-0.886,P0.05)。
[Abstract]:In order to investigate the DNA methylation pattern and mRNA expression level of muscle growth inhibitor (myostatin,MSTN) gene in muscle and fat, a 5-month-old Bashbah lamb was studied. The promoter region and methylation pattern of exon 1 of MSTN gene were detected by bisulfite sequencing method (bisulfite sequencing PCR,BSP), and real-time quantitative PCR was used to detect MSTN gene in biceps femoris, triceps femoris, semitendinosus and semimembranous muscle. The expression of mRNA in longissimus dorsi and tail fat. The results showed that the methylation probability of muscle tissue was higher than that of adipose tissue. The methylation probability of biceps femoris muscle, triceps femoris muscle, semitendinosus muscle, semimembranous muscle, longissimus dorsi muscle and tail fat were 74.2% and 83.2%, respectively, and the MSTN gene was 25.3% in biceps femoris, triceps femoris. The expression level of semitendinosus, semimembranous muscle, longissimus dorsi muscle was significantly lower than that of tail fat (P0.05), biceps femoris muscle, triceps femoris muscle, semitendinosus muscle, and semitendinosus muscle. There was no significant difference between semimembranous muscle and longissimus dorsi muscle (P0.05). The DNA methylation level of muscle and fat MSTN gene was negatively correlated with the expression of MSTN gene (r-0.886p0.05).
【作者单位】：新疆农业大学动物科学学院;
【基金】：国家自然科学基金(31260530)
【分类号】：S826

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