神经介素U在体外对猪淋巴细胞活性及自然杀伤细胞活性的影响
发布时间：2018-10-20 12:21
【摘要】：[目的]本文旨在研究神经介素U(NMU)对猪外周血淋巴细胞(PBL)的免疫调节作用。[方法]以小梅山猪为研究对象,用半定量RT-PCR方法研究了猪PBL中NMU及其受体(NMUR1和NMUR~2)基因的表达,用免疫组织化学染色方法检测了猪PBL中NMU及其受体蛋白的分布,用Cell Counting Kit-8(CCK-8)细胞计数法研究了NMU对猪PBL细胞和NK细胞活性的影响。[结果]NMUR1 mRNA在猪PBL中表达,NMU和NMUR1蛋白在猪PBL的胞膜及胞浆均有分布。用不同浓度(0.01、0.1、1、10、100和1 000 nmol·L~(-1))NMU处理体外培养的PBL,与对照组相比,0.1、1和10 nmol·L~(-1)NMU极显著提高猪PBL的细胞活性(P0.01),且当NMU浓度为10 nmol·L~(-1)时,PBL活性的水平达到峰值(179.4±3.0)%。用不同浓度(0.01、0.1、1、10、100和1 000 nmol·L~(-1))NMU处理体外培养的PBL细胞作为效应细胞,以K562细胞作为靶细胞。与对照组相比,在效靶比为15∶1时,0.1、1和10 nmol·L~(-1)的NMU均能显著提高NK细胞的细胞活性(P0.01);效靶比为30∶1时,1和10 nmol·L~(-1)的NMU均能显著提高NK细胞的细胞活性(P0.01);在效靶比为60∶1时,10 nmol·L~(-1)NMU能显著提高NK细胞的细胞活性(P0.01)。[结论]NMU及NMUR1在猪PBL中均有表达,且NMU在一定浓度范围内能提高猪NK细胞对K562细胞的杀伤能力。
[Abstract]:[objective] to study the immunomodulatory effect of interleukin U (NMU) on (PBL) in porcine peripheral blood lymphocytes. [methods] the expression of NMU and its receptor (NMUR1 and NMUR~2) genes in porcine PBL was studied by semi-quantitative RT-PCR method, and the distribution of NMU and its receptor protein in PBL was detected by immunohistochemical staining. The effects of NMU on the activity of porcine PBL and NK cells were studied by Cell Counting Kit-8 (CCK-8) cell count method. [results] NMUR1 mRNA was expressed in porcine PBL and NMU and NMUR1 proteins were distributed in the membrane and cytoplasm of porcine PBL. Compared with the control group, the cell activity of porcine PBL was significantly increased by 0.1 nmol L-1 NMU and 0.1 nmol L-1 NMU, and the PBL activity reached a peak value of (179.4 卤3.0)% when the NMU concentration was 10 nmol L-1 at different concentrations (0.01 nmol L ~ (-1) and 1 000 nmol L ~ (-1) NMU). The PBL cells cultured in vitro were treated with different concentrations (0. 01 nmol L ~ (-1) NMU) and 1 000 nmol L ~ (-1) NMU as effector cells and K562 cells as target cells. Compared with the control group, At 15:1, NMU with 0.1 and 10 nmol L-1 significantly increased the cellular activity of NK cells (P0.01), NMU with 1 and 10 nmol L-1 significantly increased the cellular activity of NK cells at 30:1, and 10 nmol L-1 NMU at 60:1. The cell activity of NK cells was significantly increased (P0.01). [conclusion] both NMU and NMUR1 are expressed in porcine PBL, and NMU can enhance the cytotoxicity of porcine NK cells to K562 cells in a certain concentration range.
【作者单位】： 南京农业大学动物医学院;
【基金】：国家自然科学基金项目(31372388)
【分类号】：S828
本文编号：2283164
[Abstract]:[objective] to study the immunomodulatory effect of interleukin U (NMU) on (PBL) in porcine peripheral blood lymphocytes. [methods] the expression of NMU and its receptor (NMUR1 and NMUR~2) genes in porcine PBL was studied by semi-quantitative RT-PCR method, and the distribution of NMU and its receptor protein in PBL was detected by immunohistochemical staining. The effects of NMU on the activity of porcine PBL and NK cells were studied by Cell Counting Kit-8 (CCK-8) cell count method. [results] NMUR1 mRNA was expressed in porcine PBL and NMU and NMUR1 proteins were distributed in the membrane and cytoplasm of porcine PBL. Compared with the control group, the cell activity of porcine PBL was significantly increased by 0.1 nmol L-1 NMU and 0.1 nmol L-1 NMU, and the PBL activity reached a peak value of (179.4 卤3.0)% when the NMU concentration was 10 nmol L-1 at different concentrations (0.01 nmol L ~ (-1) and 1 000 nmol L ~ (-1) NMU). The PBL cells cultured in vitro were treated with different concentrations (0. 01 nmol L ~ (-1) NMU) and 1 000 nmol L ~ (-1) NMU as effector cells and K562 cells as target cells. Compared with the control group, At 15:1, NMU with 0.1 and 10 nmol L-1 significantly increased the cellular activity of NK cells (P0.01), NMU with 1 and 10 nmol L-1 significantly increased the cellular activity of NK cells at 30:1, and 10 nmol L-1 NMU at 60:1. The cell activity of NK cells was significantly increased (P0.01). [conclusion] both NMU and NMUR1 are expressed in porcine PBL, and NMU can enhance the cytotoxicity of porcine NK cells to K562 cells in a certain concentration range.
【作者单位】： 南京农业大学动物医学院;
【基金】：国家自然科学基金项目(31372388)
【分类号】：S828
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，本文编号：2283164
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