昆明市西山区山羊伪结核棒状杆菌的分离鉴定

发布时间：2018-10-20 16:59

【摘要】：羊伪结核病是山羊一种慢性消耗性细菌病,对山羊养殖业的危害极大。为控制本病的发生,从2016年期间,对昆明市西山区存栏的38 225只山羊开展流行病学调查,结果表明伪结核病在西山区山羊平均发病率为10.3%,病死率0.1%,采集病料经细菌分离鉴定、小鼠毒力试验、16SrRNA序列分析比较及药敏试验,结果表明,引起本病的病原菌鉴定为山羊伪结核棒状杆菌,对其中2个菌株完成了生化鉴定及药敏试验分析。生化及16S序列分析结果表明,分离菌株为羊伪结核棒状杆菌,对完成系统鉴定的2株细菌完成药敏试验,结果表明,除链霉素、甲硝唑及复方新诺明外的大多数抗菌药物均对其敏感,可为该地区及昆明其他疫区临床用药提供参考。
[Abstract]:Goat pseudo-tuberculosis is a chronic expendable bacterial disease in goats, which is harmful to goat breeding. In order to control the occurrence of this disease, an epidemiological investigation was carried out on 38 225 goats in Xishan District of Kunming City during 2016. The results showed that the average incidence of pseudotuberculosis in goats in Xishan District was 10.3, the mortality rate was 0.1%, and the samples were isolated and identified by bacteria. The virulence test, 16SrRNA sequence analysis and drug sensitivity test showed that the pathogen causing the disease was identified as Corynebacterium pseudotuberculosis in goat, and two of the strains were identified by biochemical identification and drug sensitivity test. The results of biochemistry and 16s sequence analysis showed that the isolated strain was Corynebacterium tuberculosis pseudotuberculosis of sheep. The drug sensitivity test was completed for 2 strains that had completed the systematic identification. The results showed that streptomycin was removed. Most antimicrobial agents of metronidazole and compound sulfamethoxazole are sensitive to metronidazole, which can provide reference for clinical use of metronidazole and other epidemic areas in Kunming.
【作者单位】：昆明市西山区农林局;云南省畜牧兽医科学院;云南农业大学;昆明市西山区海口街道办事处;
【基金】：昆明市西山区2016年度科技项目国家现代肉羊产业技术体系项目(CARS-39)
【分类号】：S858.27

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