猪卵巢发育的组织学变化及卵泡闭锁规律研究

发布时间：2018-10-21 18:39

【摘要】：旨在对不同日龄猪卵巢组织结构以及卵巢发育过程中卵泡闭锁规律进行研究。取3、40、50、60、72、86、95及165日龄猪卵巢各3例,采用常规石蜡切片、HE染色和TUNEL技术检测卵巢组织结构及卵泡闭锁规律,结果表明,猪卵巢发育从组织学上可分为卵原细胞增殖期、卵泡缓慢生长期及卵泡快速生长期3个阶段。卵原细胞增殖期主要以卵原细胞的增殖分裂为特点;在卵泡缓慢生长期,原始卵泡的数量随日龄增加逐渐减少,初级卵泡数量及体积则明显增加;至卵泡快速生长期,生长卵泡的数量及体积继续增大,其数量在86日龄达到最大值;72日龄卵巢中出现三级卵泡,至95日龄卵巢中出现近成熟卵泡。在各时期的卵巢组织,均可观察到卵原细胞及卵泡的闭锁现象,以原始卵泡的退化最为显著。TUNEL检测表明,在卵原细胞增殖期,可见大量原始卵泡及初级卵泡的闭锁,其闭锁主要源自卵泡卵母细胞的凋亡。在卵泡缓慢生长期,各级生长卵泡均可出现闭锁,初级卵泡的闭锁主要由卵母细胞的凋亡引起,也有部分是同时伴有卵母细胞和卵泡细胞凋亡的;次级卵泡的闭锁则主要由于颗粒层细胞的凋亡所致。在卵泡快速生长期,随着卵泡的快速生长,各级卵泡的闭锁也变得更加明显,次级卵泡及三级卵泡的闭锁主要是由颗粒细胞凋亡引起。
[Abstract]:To study the ovarian tissue structure and follicular atresia during ovarian development in pigs of different day ages. The ovarian tissue structure and follicular atresia were detected by routine paraffin section, HE staining and TUNEL technique. The results showed that the development of pig ovary could be divided into oocyte proliferative phase histologically. Follicular slow growth stage and follicular rapid growth stage 3 stages. At the stage of slow follicle growth, the number of primordial follicles gradually decreased, but the number and volume of primary follicles increased obviously. The number and volume of the growth follicles continued to increase, and the number reached the maximum at 86 days old, and the third follicles appeared in the 72-day-old ovaries and the nearly mature follicles in the 95-day-old ovaries. The atresia of oocyte and follicle was observed in all stages of ovarian tissue, especially the degeneration of primordial follicle. TUNEL showed that a large number of primordial follicles and primary follicles were atresia during the proliferation of oocyte. Its atresia is mainly derived from the apoptosis of follicular oocytes. At the stage of slow follicle growth, all growth follicles appear atresia. The primary follicle atresia is mainly caused by the apoptosis of oocytes, some of which are accompanied by apoptosis of oocytes and follicular cells. The atresia of secondary follicles was mainly due to the apoptosis of granular layer cells. In the period of rapid follicle growth, the atresia of all follicles becomes more obvious with the rapid growth of follicles. The atresia of secondary follicles and tertiary follicles is mainly caused by the apoptosis of granulosa cells.
【作者单位】：西北农林科技大学动物医学院;
【基金】：陕西省科技攻关项目(2014K02-05-01) 西北农林科技大学2015年第二批基本科研业务费专项资金项目(2452015157)
【分类号】：S828

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