GnRH主动免疫对雄性藏绵羊血液中甲状腺激素含量的影响

发布时间：2018-10-23 16:56

【摘要】：为探讨GnRH并列体二聚物(GnRH-TDK)对雄性藏绵羊甲状腺功能的影响,采用GnRH-TDK主动免疫雄性藏绵羊,并将雄性藏绵羊随机分为免疫去势、手术去势及对照3个组,其中免疫去势组于25周龄时初免,8周后加免;手术去势组在试验开始前1周进行外科手术去势;对照组不做任何处理。以初免当天为0周,每4周采集血样1次,加免后8周屠宰所有试验羊并分离两侧睾丸,应用酶联免疫法(ELISA)测定血清抗体滴度及LH、FSH、T、TSH、T3和T4含量变化。结果表明,免疫去势组绵羊产生了良好的抗体反应,初免及加免后体内抗GnRH抗体滴度均快速升高,且血清T含量显著下降(P0.05),至屠宰时仍维持在较低水平(4.27 ng·m L~(-1)),同时睾丸严重萎缩。与对照组相比,免疫去势组血清TSH、T3及T4未显著下降。由此可见,GnRH主动免疫对血清中TSH、T3及T4浓度无明显影响,即GnRH主动免疫对雄性藏绵羊甲状腺功能无影响。本研究结果为GnRH主动免疫去势的分子机制以及对机体的影响奠定了理论基础。
[Abstract]:In order to investigate the effect of GnRH dimer (GnRH-TDK) on thyroid function of male Tibetan sheep, male Tibetan sheep were immunized with GnRH-TDK. The male Tibetan sheep were randomly divided into three groups: immune castration group, surgical castration group and control group. The immune castration group was first immunized at the age of 25 weeks and added after 8 weeks. The castration group underwent surgical castration one week before the beginning of the experiment; the control group did not do any treatment. The blood samples were collected once every 4 weeks on the day of the first immunization. All the sheep were slaughtered and the testis were separated 8 weeks after immunization. The serum antibody titers and the changes of LH,FSH,T,TSH,T3 and T4 contents were determined by (ELISA). The results showed that the ovariectomized sheep had a good antibody response, and the titers of anti GnRH antibody increased rapidly in both the primary and the added groups. The serum T content decreased significantly (P0.05), and remained at a low level (4.27 ng mL ~ (-1),) at the slaughter stage, with severe testicular atrophy. Compared with the control group, the serum TSH,T3 and T 4 levels in the castrated group were not significantly decreased. It can be seen that GnRH active immunization has no effect on serum TSH,T3 and T4 concentration, that is, GnRH active immunization has no effect on thyroid function of male Tibetan sheep. The results of this study provide a theoretical basis for the molecular mechanism of active immune castration of GnRH and its effect on the body.
【作者单位】：四川农业大学生命科学学院原子能农业应用研究室;西藏自治区农牧科学院畜牧兽医研究所;中国农业科学院北京畜牧兽医研究所/农业部畜禽遗传资源与种质创新重点实验室;
【基金】：四川省教育厅重点项目(15ZA0005) 西藏自治区科技厅自然基金中国农业科学院科技创新工程(ASTIP-IAS13)
【分类号】：S826

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