重组大熊猫IFN-γ聚氰基丙烯酸正丁酯纳米球的制备与评价
发布时间：2018-10-24 11:39
【摘要】：为了制备并评价大熊猫(Ailuropoda melanoleuca)重组干扰素γ(IFN-γ)聚氰基丙烯酸正丁酯(PBCA)纳米球,本研究利用蛋白质原核表达技术,获得了重组大熊猫IFN-γ蛋白,然后以PBCA为载药材料,采用乳化聚合法制备了大熊猫干扰素γ纳米微球(IFNγ-PBCA-NS),最后借助感染大熊猫流感病毒A/Panda/Sichuan/01/2011(H1N1)的昆明小鼠(Mus musculus)模型,通过灌胃和皮下注射药物初步评价了IFNγ-PBCA-NS的药效。结果表明,大熊猫IFN-γ的蛋白分子量约为33.5 ku,所制备的大熊猫IFNγ-PBCA-NS外观规整,粒径在50~200 nm之间,跨距0.55,大小较均匀,包封率为56.7%,载药量为0.86%。灌胃和注射两种给药途径中,IFNγ-PBCA-NS组小鼠的生命延长率均显著高于IFN-γ组(P0.05或P0.01)。显示IFNγ-PBCA-NS在小鼠体内有更好的缓释和抗病毒作用,可为进一步研究制备大熊猫多肽类药物微粒提供参考。
[Abstract]:In order to prepare and evaluate giant panda (Ailuropoda melanoleuca) recombinant interferon 纬 (IFN- 纬) polybutyl cyanoacrylate (PBCA) nanospheres, the recombinant giant panda IFN- 纬 protein was obtained by using prokaryotic protein expression technique, and then PBCA was used as drug carrier. Giant panda interferon 纬 nanospheres (IFN 纬-PBCA-NS) were prepared by emulsion polymerization. Finally, the drug efficacy of IFN 纬-PBCA-NS was evaluated by intragastric administration and subcutaneous injection of IFN 纬-PBCA-NS by (Mus musculus) model of Kunming mice infected with giant panda influenza virus A/Panda/Sichuan/01/2011 (H1N1). The results showed that the giant panda IFN 纬-PBCA-NS prepared by the giant panda IFN- 纬 with molecular weight of about 33. 5 ku, had a regular appearance, the diameter was between 50 ~ 200 nm, the span was 0. 55, the size was uniform, the entrapment efficiency was 56. 7, and the drug loading was 0. 86%. The prolongation rate of life in IFN 纬 PBCA-NS group was significantly higher than that in IFN- 纬 group (P0.05 or P0.01). The results showed that IFN 纬-PBCA-NS had better sustained release and antiviral effects in mice, which could provide a reference for further study on the preparation of giant panda polypeptide drug particles.
【作者单位】： 四川农业大学生命科学学院;四川农业大学动物生物技术中心;中国保护大熊猫研究中心;
【基金】：四川省科技计划项目(No.2012JQ0037)
【分类号】：S859.5
[Abstract]:In order to prepare and evaluate giant panda (Ailuropoda melanoleuca) recombinant interferon 纬 (IFN- 纬) polybutyl cyanoacrylate (PBCA) nanospheres, the recombinant giant panda IFN- 纬 protein was obtained by using prokaryotic protein expression technique, and then PBCA was used as drug carrier. Giant panda interferon 纬 nanospheres (IFN 纬-PBCA-NS) were prepared by emulsion polymerization. Finally, the drug efficacy of IFN 纬-PBCA-NS was evaluated by intragastric administration and subcutaneous injection of IFN 纬-PBCA-NS by (Mus musculus) model of Kunming mice infected with giant panda influenza virus A/Panda/Sichuan/01/2011 (H1N1). The results showed that the giant panda IFN 纬-PBCA-NS prepared by the giant panda IFN- 纬 with molecular weight of about 33. 5 ku, had a regular appearance, the diameter was between 50 ~ 200 nm, the span was 0. 55, the size was uniform, the entrapment efficiency was 56. 7, and the drug loading was 0. 86%. The prolongation rate of life in IFN 纬 PBCA-NS group was significantly higher than that in IFN- 纬 group (P0.05 or P0.01). The results showed that IFN 纬-PBCA-NS had better sustained release and antiviral effects in mice, which could provide a reference for further study on the preparation of giant panda polypeptide drug particles.
【作者单位】： 四川农业大学生命科学学院;四川农业大学动物生物技术中心;中国保护大熊猫研究中心;
【基金】：四川省科技计划项目(No.2012JQ0037)
【分类号】：S859.5
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