内蒙古东部地区奶牛布鲁氏菌病流行学调查及分离与鉴定

发布时间：2018-10-25 16:05

【摘要】：为掌握内蒙古自治区东部地区奶牛布鲁氏菌病的流行现状和流行特征,在试验中采用全乳环状试验(milk ring test,简称MRT)、奶液间接酶联免疫吸附试验(indire enzyme-linked immunosorbent assay,简称iELISA)两种方法对科尔沁地区5个牛场进行了布病流行学调查,比较两种方法对检测布鲁氏菌病阳性牛的差异性。通过对内蒙古自治区东部地区5个盟市进行牛布鲁氏菌病流行学调查;最后对检出的部分阳性奶牛乳汁进行细菌分离培养,用VirB8和AMOS方法进行扩增鉴定。表明两种方法(MRT和iELISA)检测结果差异不显著;经检测17株分离株均为布鲁氏菌。布鲁氏菌分子分型扩增的鉴定显示,牛场感染的菌株为牛种布鲁氏菌。内蒙古东部地区部分饲养场布鲁氏菌病阳性率为1.67%。
[Abstract]:In order to understand the epidemic status and characteristics of brucellosis in dairy cattle in the eastern part of Inner Mongolia Autonomous region, The whole milk ring test (milk ring test,) and MRT), milk indirect enzyme linked immunosorbent assay (indire enzyme-linked immunosorbent assay, iELISA) were used to investigate the prevalence of brucellosis in five cattle farms in Horqin area. To compare the difference between the two methods in the detection of brucellosis positive cattle. Through the epidemiological investigation of bovine brucellosis in five cities in the eastern part of Inner Mongolia Autonomous region, the milk of some positive cows was isolated and cultured, and amplified and identified by VirB8 and AMOS. The results showed that there was no significant difference between the two methods (MRT and iELISA), and 17 strains of Brucella were detected. The results of molecular typing and amplification of Brucella showed that the strain infected in cattle farm was Brucella bovis. The positive rate of brucellosis in some farms in eastern Inner Mongolia was 1.67.
【作者单位】：内蒙古民族大学动物科学技术学院;吉林大学动物科学学院;
【基金】：国家自然科学基金项目(31260608) 内蒙古自治区高等学校科学技术研究重点项目(NJZZ12117) 内蒙古通辽市校科技合作项目(SXZD2012131) 内蒙古自治区自然科学基金项目(2015MS0339)共同资助
【分类号】：S858.23

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