副鸡禽杆菌感染对鸡鼻区免疫相关因子的影响

发布时间：2018-10-29 21:35

【摘要】：副鸡禽杆菌是巴氏杆菌科的一种短小革兰阴性菌,也是鸡传染性鼻炎的致病菌。为探讨副鸡禽杆菌感染后靶器官免疫相关因子的变化情况,用副鸡禽杆菌进行人工感染SPF试验鸡,分别在第3天和第6天采集鼻区组织,应用RT-qPCR方法检测了IL-1β、IL-6、IL-8、IFN-γ、IL-4、IL-10、TLR4、TLR5、TLR15、AvβDs 4和AvβDs 10的相对表达量;收集鼻黏膜洗液,检测sIgA水平。结果显示,感染组试验鸡IL-1β、IL-6、IL-8、IFN-γ、IL-4、IL-10、TLR4、TLR5、TLR15、β-防御素4、10相对表达量显著上升;鼻腔黏膜sIgA显著增高。提示前炎因子、趋化因子、Th1细胞因子、Th2细胞因子都参与了对副鸡禽杆菌感染的免疫调控,分泌的sIgA在抗副鸡禽杆菌感染过程中也起到重要作用。本研究探索了副鸡禽杆菌感染后细胞因子及抗体变化,对探寻新的免疫或治疗方法奠定了基础。
[Abstract]:Avian Paralyx is a short Gram-negative bacteria of Pasteurella and a pathogenic bacteria of infectious rhinitis in chicken. In order to investigate the changes of immune related factors in target organs after infection of Avian Paralyx, SPF 尾 was detected by RT-qPCR method in the nasal area of chickens infected with Avian Paralyx on day 3 and day 6, respectively. The relative expression of IL-6,IL-8,IFN- 纬, IL-4,IL-10,TLR4,TLR5,TLR15,Av 尾 Ds 4 and Av 尾 Ds 10; Nasal lavage fluid was collected and sIgA level was detected. The results showed that the relative expression of IL-1 尾, IL-6,IL-8,IFN- 纬, IL-4,IL-10,TLR4,TLR5,TLR15, 尾 -defensin 4 and 10 in infected chickens were significantly increased, while sIgA in nasal mucosa was significantly increased. These results suggest that proinflammatory factor, chemokine, Th1 cytokine and Th2 cytokine are all involved in the immune regulation of Avian Paralyx infection, and the secreted sIgA also plays an important role in the process of anti-Avian infection. In this study, the changes of cytokines and antibodies after infection of Avian Paralyx were explored, which laid a foundation for exploring new immunological or therapeutic methods.
【作者单位】：北京市农林科学院畜牧兽医研究所畜禽疫病防控技术北京市重点实验室;沈阳农业大学生物科学与技术学院;
【基金】：国家自然科学基金项目(31272558) 农业部公益性行业专项(201303044) 北京市农林科学院创新能力建设项目(KJCX20140410)
【分类号】：S858.31

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