牛种布鲁氏菌RpoE2蛋白部分生物学特性研究

发布时间：2018-10-30 11:33

【摘要】：为探讨牛种布鲁氏菌sigma因子RpoE2的功能,采用酵母双杂交技术对RpoE2蛋白与anti-sigma因子之间的互作进行研究;通过环境应激试验评价了rpoE2缺失株对强氧化环境、酸性pH、杀菌性阳离子多肽、铁缺乏环境的耐受能力;通过比较亲本菌株和缺失株在巨噬细胞感染模型和小鼠感染模型中的生存能力,研究了rpoE2基因是否与牛种布鲁氏菌毒力相关。结果显示:牛种布鲁氏菌的RpoE2蛋白可以与anti-sigma因子相互作用,牛种布鲁氏菌rpoE2基因缺失后,对各种应激环境的耐受能力均无影响,并且巨噬细胞和小鼠感染模型中毒力均不降低。牛种布鲁氏菌的RpoE2蛋白是一个ECF16家族的sigma因子,并且其与布鲁氏菌的毒力无关。
[Abstract]:In order to study the function of sigma factor RpoE2 in bovine brucella, the interaction between RpoE2 protein and anti-sigma factor was studied by yeast two-hybrid technique. Environmental stress test was used to evaluate the tolerance of rpoE2 deficient strain to strong oxidative environment, acidic pH, bactericidal cationic polypeptide, and iron deficiency. By comparing the viability of parent strain and deletion strain in macrophage infection model and mouse infection model, the relationship between rpoE2 gene and brucella virulence was studied. The results showed that the RpoE2 protein of Brucella bovis could interact with the anti-sigma factor, and the rpoE2 gene deletion of Brucella spp had no effect on the tolerance of various stress environments. The toxicity of macrophages and mice was not decreased. The RpoE2 protein of bovine brucella is a sigma factor of ECF16 family, and it is not related to the virulence of brucella.
【作者单位】：中国农业大学动物医学院;中国动物疫病预防控制中心;乳山市夏村镇畜牧兽医工作站;中国兽医药品监察所;
【基金】：国家973项目(2010CB530202) 国家自然科学基金资助项目(30871882)
【分类号】：S852.61

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