犬圆环病毒及犬细小病毒双重PCR检测方法的建立
发布时间：2018-10-31 10:10
【摘要】：为建立能同时检测犬圆环病毒(CanineCV)和犬细小病毒(CPV)的方法,根据CanineCV和CPV基因组的保守区域设计2对特异性引物,预期特异性扩增CanineCV和CPV的片段大小分别为984和668 bp。并将反应条件进行优化,建立了CanineCV和CPV的双重PCR检测方法。特异性检测结果显示,该方法能特异性扩增CanineCV和CPV的目的条带,且未扩增出其他犬胃肠道病原;CanineCV和CPV的最低检出限分别为85.7和312 copies/L。对采集自广西地区的223份临床样品的检测结果显示,CanineCV的阳性率为2.24%(5/223)、CPV的阳性率为5.38%(12/223),后者与常规PCR检测方法相一致。结果表明,建立的双重PCR方法可以用于CanineCV和CPV的检测和流行病学调查。
[Abstract]:In order to establish a method for simultaneous detection of canine circovirus (CanineCV) and canine parvovirus (CPV), two pairs of specific primers were designed according to the conserved region of CanineCV and CPV genomes. The expected length of CanineCV and CPV fragments were 984 and 668 bp., respectively. The reaction conditions were optimized and a dual PCR detection method for CanineCV and CPV was established. The results of specific detection showed that the target bands of CanineCV and CPV could be amplified by this method, and the detection limits of CanineCV and CPV were 85.7 and 312 copies/L., respectively. The positive rate of CanineCV was 2.24% (5 / 223), CPV, 5.38%, 12 / 223), which was consistent with the conventional PCR method. The results showed that the dual PCR method could be used for the detection and epidemiological investigation of CanineCV and CPV.
【作者单位】： 吉林农业大学动物科学技术学院;军事医学科学院军事兽医研究所;
【基金】：国家自然科学基金资助项目(31272573)
【分类号】：S852.655
[Abstract]:In order to establish a method for simultaneous detection of canine circovirus (CanineCV) and canine parvovirus (CPV), two pairs of specific primers were designed according to the conserved region of CanineCV and CPV genomes. The expected length of CanineCV and CPV fragments were 984 and 668 bp., respectively. The reaction conditions were optimized and a dual PCR detection method for CanineCV and CPV was established. The results of specific detection showed that the target bands of CanineCV and CPV could be amplified by this method, and the detection limits of CanineCV and CPV were 85.7 and 312 copies/L., respectively. The positive rate of CanineCV was 2.24% (5 / 223), CPV, 5.38%, 12 / 223), which was consistent with the conventional PCR method. The results showed that the dual PCR method could be used for the detection and epidemiological investigation of CanineCV and CPV.
【作者单位】： 吉林农业大学动物科学技术学院;军事医学科学院军事兽医研究所;
【基金】：国家自然科学基金资助项目(31272573)
【分类号】：S852.655
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