妊娠后期营养限制对母羊胃肠道葡萄糖转运载体相关基因表达的影响
发布时间：2018-11-01 16:42
【摘要】：本研究旨在研究妊娠后期营养限制对母羊胃肠道葡萄糖转运载体相关基因表达的影响。选取20只同期受孕的湘东黑山羊,随机分为2组,即对照组(自由采食)和限饲组(40%采食量限制),每组10只。预试期15 d(妊娠81~95 d),正试期39 d(妊娠96~135 d)。正试期结束后,屠宰并采取瘤胃、十二指肠、空肠、回肠以及盲肠的黏膜样品,利用实时定量PCR技术,检测Na+-葡萄糖共转运载体1(SGLT1)、Na+-葡萄糖共转运载体3(SGLT3)、易化葡萄糖转运载体2(GLUT2)和易化葡萄糖转运载体5(GLUT5)基因表达量。结果表明:限饲组与对照组相比,SGLT1基因表达量在瘤胃显著降低(P0.05),在空肠和回肠中有降低趋势(0.05≤P0.10);GLUT5基因表达量在盲肠显著降低(P0.05);而其他葡萄糖转运载体基因胃肠道表达量在限饲组和对照组差异均不显著(P0.05)。由此可见,母羊妊娠后期营养限制对胃肠道中葡萄糖转运载体基因表达有不同程度的影响,进而引起母羊机体内葡萄糖转运的改变。
[Abstract]:The aim of this study was to investigate the effects of nutritional restriction on the expression of glucose transporter related genes in the gastrointestinal tract of ewe. Twenty Xiangdong black goats who were pregnant at the same time were randomly divided into two groups: the control group (free feed intake) and the restricted feeding group (40% feed intake restriction), with 10 goats in each group. Pretest period 15 days (pregnancy 81: 95 d),) 39 days (pregnancy 96135 d).) The mucosal samples of rumen, duodenum, jejunum, ileum and cecum were slaughtered at the end of the trial period. Na glucose co-transport vector 1 (SGLT3) was detected by real-time quantitative PCR. The gene expression of glucose transport vector 2 (GLUT2) and glucose transport vector 5 (GLUT5) was facilitated. The results showed that compared with the control group, the expression of SGLT1 gene decreased significantly in rumen (P0.05), decreased in jejunum and ileum (0.05 鈮，
本文编号：2304499
[Abstract]:The aim of this study was to investigate the effects of nutritional restriction on the expression of glucose transporter related genes in the gastrointestinal tract of ewe. Twenty Xiangdong black goats who were pregnant at the same time were randomly divided into two groups: the control group (free feed intake) and the restricted feeding group (40% feed intake restriction), with 10 goats in each group. Pretest period 15 days (pregnancy 81: 95 d),) 39 days (pregnancy 96135 d).) The mucosal samples of rumen, duodenum, jejunum, ileum and cecum were slaughtered at the end of the trial period. Na glucose co-transport vector 1 (SGLT3) was detected by real-time quantitative PCR. The gene expression of glucose transport vector 2 (GLUT2) and glucose transport vector 5 (GLUT5) was facilitated. The results showed that compared with the control group, the expression of SGLT1 gene decreased significantly in rumen (P0.05), decreased in jejunum and ileum (0.05 鈮，
本文编号：2304499
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