家兔肠道微生物ERIC-PCR指纹图谱构建及分析

发布时间：2018-11-02 19:48

【摘要】：【目的】构建不同周龄健康家兔及腹泻家兔肠道微生物的ERIC-PCR指纹图谱,分析二者肠道菌群结构的多样性及其差异性,为优化家兔饲养和疾病防治打下基础。【方法】收集1~8周龄健康家兔与腹泻家兔粪便样本,分离肠道微生物并提取其基因组DNA,PCR扩增其16S r DNA与ERIC,构建ERIC-PCR指纹图谱,对不同周龄健康家兔及腹泻家兔肠道菌群结构进行分析。【结果】肠道微生物迅速在1周龄仔兔肠道中定植且总量达最高值,其后不断减少,5周龄后总量趋于稳定,且1周龄仔兔与5周龄后(断奶后)家兔的肠道微生物总量间均存在显著差异(P0.05)。随着周龄的增加,肠道微生物ERIC条带特征发生明显变化,各周龄出现了不同的特征性ERIC条带。与同一周龄健康家兔相比,腹泻家兔肠道微生物ERIC条带明显减少,条带特征存在明显差异,菌群多样性低于健康家兔。【结论】随着周龄的增加,健康家兔的肠道微生物菌群结构和多样性发生明显变化。与同一周龄健康家兔相比,腹泻家兔肠道微生物菌群结构明显改变,多样性明显降低,且出现健康家兔中未出现过的优势菌群,进一步证明肠道微生物菌群结构与家兔生长发育和腹泻存在关联性。
[Abstract]:[objective] to construct the ERIC-PCR fingerprint of intestinal microbes of healthy rabbits and rabbits with diarrhea at different weeks of age, and to analyze the diversity and difference of intestinal flora between them. In order to optimize the breeding and disease prevention of rabbits, the fecal samples of 1-week old healthy rabbits and rabbits with diarrhea were collected, the intestinal microbes were isolated and their genomic DNA,PCR was extracted to amplify their 16s r DNA and ERIC, to construct the ERIC-PCR fingerprint. The intestinal flora structure of healthy rabbits and diarrhea rabbits at different weeks was analyzed. [results] intestinal microbes were rapidly colonized in the intestine of 1-week-old rabbits and reached the highest value, then decreased continuously, and the total amount tended to be stable after 5 weeks of age. There were significant differences in total intestinal microorganism between 1-week-old rabbits and 5-week-old rabbits (P0.05). With the increase of age, the ERIC bands of intestinal microbes changed obviously, and different characteristic ERIC bands appeared in each week. Compared with the healthy rabbits of the same week, the ERIC bands of intestinal microbes in the diarrhea rabbits were obviously decreased, and the characteristics of the bands were significantly different, and the diversity of bacteria was lower than that of the healthy rabbits. [conclusion] with the increase of the age of the week, the bacterial diversity of diarrhea rabbits is lower. The structure and diversity of intestinal microflora in healthy rabbits were obviously changed. Compared with the healthy rabbits of the same week, the intestinal microflora structure of the diarrhea rabbits was obviously changed, the diversity was obviously decreased, and the dominant flora was found in the healthy rabbits. It was further demonstrated that the structure of intestinal microflora was related to the growth and development of rabbits and diarrhea.
【作者单位】：四川理工学院制药工程系;
【基金】：四川理工学院人才引进项目(2011RC16)
【分类号】：S858.291

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