无乳链球菌荚膜多糖的粗提及多糖含量测定条件优化
发布时间：2018-11-05 09:19
【摘要】：为提取无乳链球菌荚膜多糖,并对苯酚-硫酸法测定多糖含量条件进行优化。离心收集发酵上清液,超滤浓缩,经800mL/L乙醇沉淀提取无乳链球菌荚膜多糖;采用单因素试验对苯酚硫酸法测定条件进行优化,并对测定方法的可靠性进行验证。结果表明,从1L发酵液中提取到含量为38.51%的荚膜多糖0.36g;测定的最佳条件为:2mL样品液中,加入1 mL 50 mL/L的苯酚,5 mL浓硫酸,混匀后80℃作用20min,在1.5h内测定485nm处的光密度。在该条件下,平均加样回收率为99.96%,相对标准偏差为0.40%。说明从无乳链球菌发酵上清液中成功提取到荚膜多糖,建立了苯酚硫酸法测定荚膜多糖的最优条件。
[Abstract]:In order to extract the capsular polysaccharides from Streptococcus lactis and optimize the conditions for the determination of polysaccharides by phenol-sulfuric acid method. The supernatant of fermentation was collected by centrifugation and concentrated by ultrafiltration. The capsule polysaccharide of Streptococcus acuminatum was extracted by 800mL/L ethanol precipitation. The conditions of phenol sulfuric acid method were optimized by single factor test and the reliability of the method was verified. The results showed that the content of capsule polysaccharides was 38.51% and 0.36 g from 1L fermentation broth. The optimum conditions were as follows: 1 mL 50 mL/L phenol and 5 mL concentrated sulfuric acid were added to 2mL sample solution, then mixed at 80 鈩，
本文编号：2311622
[Abstract]:In order to extract the capsular polysaccharides from Streptococcus lactis and optimize the conditions for the determination of polysaccharides by phenol-sulfuric acid method. The supernatant of fermentation was collected by centrifugation and concentrated by ultrafiltration. The capsule polysaccharide of Streptococcus acuminatum was extracted by 800mL/L ethanol precipitation. The conditions of phenol sulfuric acid method were optimized by single factor test and the reliability of the method was verified. The results showed that the content of capsule polysaccharides was 38.51% and 0.36 g from 1L fermentation broth. The optimum conditions were as follows: 1 mL 50 mL/L phenol and 5 mL concentrated sulfuric acid were added to 2mL sample solution, then mixed at 80 鈩，
本文编号：2311622
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