大围山微型鸡、云龙矮脚鸡和拉伯高脚鸡mtDNA D-loop遗传多样性分析

发布时间：2018-11-05 10:42

【摘要】：大围山微型鸡、云龙矮脚鸡和拉伯高脚鸡是近年在云南发现的地方鸡遗传资源,具有优良的种质特性,为了阐明其群体遗传背景,采用mtDNA D-loop区序列为遗传标记,对采自3个地方鸡的共257个样品进行了检测分析。结果表明:大围山微型鸡、云龙矮脚鸡和拉伯高脚鸡的单倍型多样度分别为0.083±0.018、0.851±0.029和0.784±0.039;核苷酸多样度分别为0.014 67±0.000 45、0.013 42±0.000 80、0.011 66±0.001 55;群体内遗传距离分别为0.014±0.003、0.014±0.003、0.012±0.003。中介网络分析显示:本研究检测的257个样品分布在A、B、C、D、E、F和G等7个世系中,其中大围山微型鸡包含A、B、C、D、E、F和G等7个世系,B、D世系为其主要世系;云龙矮脚鸡包含A、B、C、E、F和G等6个世系,E世系为其主要世系;拉伯高脚鸡包含A、B、E和G等4个世系,G世系为其主要世系。AMOVA分析表明:3个群体间遗传分化指数(Fst)分别为0.120 84、0.239 46、0.263 68(P0.01),群体内遗传变异占80.61%,群体间变异为19.39%(P0.01)。本研究结果揭示3个地方鸡群体遗传多样性较丰富,都含有多个母系血统,支持家鸡多个母系起源的观点,但它们之间存在较大的遗传分化。
[Abstract]:In order to elucidate the population genetic background, mtDNA D-loop region sequence was used as a genetic marker to clarify the population genetic background of miniature chicken, Yunlong dwarf chicken and Rabbo Gaoxiao chicken, which were found in Yunnan in recent years and have excellent germplasm characteristics. A total of 257 samples collected from 3 local chickens were detected and analyzed. The results showed that the haplotypes were 0.083 卤0.018, 0.851 卤0.029 and 0.784 卤0.039, respectively. The nucleotide diversity was 0.014 67 卤0.000 450.13 42 卤0.000 80,0.011 66 卤0.001 55, and the genetic distance within population was 0.014 卤0.003 0. 014 卤0. 003 0. 012 卤0. 003 respectively. The analysis of intermediary network showed that 257 samples were distributed in 7 lineages, such as AZB, DX, E, F and G, among which 7 lineages, B, were included in the miniature chicken of Daweishan, I. e., AZB, C, D, E, F and G, B, B, D-lineage is the main lineage; Yunlong Dwarf Chicken consists of 6 lineages, such as AZB, C, E, and G, and E is the main lineage. The AMOVA analysis showed that the genetic differentiation index (Fst) of the three populations was 0.120 ~ (84) (0.239,466) and 0.263.68 (P0.01) respectively, and the genetic differentiation index (Fst) of the three populations was 0.239,0.263.The genetic differentiation index of the three populations was 0.239,0.263.The genetic differentiation index (Fst) of the three populations was 0.239,0.263 68 (P0.01). Genetic variation within population accounted for 80.61% (P 0.01) and variation among populations was 19.39% (P0.01). The results of this study revealed that the genetic diversity of the three local chicken populations was abundant, and all of them contained multiple maternal lineages, which supported the idea of the origin of multiple maternal lines of domestic chickens, but there was a large genetic differentiation among them.
【作者单位】：云南农业大学动物科学技术学院;云南农业职业技术学院畜牧兽医系;云南省家畜改良工作站;云南农业大学农科专业基础实验教学示范中心;
【基金】：云南省教育厅科学研究基金项目(5Y0196B,0112340) 国家自然科学基金项目(31460582,30660024) 云南省应用基础研究重点项目(2014FA032,2007C0003Z)
【分类号】：S831.2

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