葛芪复方对仔猪大肠杆菌性腹泻作用效果研究
发布时间：2018-11-07 11:06
【摘要】：葛芪复方是中国农业科学院上海兽医研究所药学研究室自主筛选的新型复方,该复方根据中兽医辩证施治理论,具有良好的抗腹泻作用效果。为了评价该复方的作用效果,进行了临床前药效评价试验,包括复方对小鼠大肠杆菌性腹泻作用效果研究,仔猪大肠杆菌性腹泻病理模型研究,复方对仔猪大肠杆菌性腹泻作用效果研究以及复方及几味单药对大肠杆菌体外抑菌效果研究,为葛芪复方新药研究提供试验资料。1复方对小鼠大肠杆菌性腹泻作用效果研究通过预先建立小鼠大肠杆菌性腹泻病理模型,设计0.3mL/10g药物组、0.2mL/10g药物组、0.1mL/10g药物组、模型对照组和空白对照组,连续灌服给药7d,观察复方对腹泻小鼠的防治效果。结果表明,给药后各剂量组小鼠平均增重、血液学指标、脾和胸腺脏器指数与空白对照组无显著差异,与模型对照组差异明显(P0.05)。十二指肠组织病理学观察各剂量组没有发现明显病变,模型对照组肠粘膜绒毛肿胀变粗,由此说明该复方能够有效防治小鼠腹泻。2仔猪大肠杆菌性腹泻病理模型研究通过设计5mL/10Kg感染组、2.5mL/10Kg感染组、1mL/10Kg感染组和空白对照组,各感染组仔猪通过口腔灌服相应的3×109CFU/mL大肠杆菌菌悬液,连续3d。结果表明,高剂量组、中剂量组仔猪平均增重、细菌学检查、血液学指标及电解质指标与空白对照组差异显著(P0.05)。由此说明,通过口腔灌服3×109CFU/mL大肠杆菌菌悬液方式,按照5mL/10Kg剂量,连续3d,可以有效建立仔猪大肠杆菌性腹泻模型。3复方对仔猪大肠杆菌性腹泻作用效果研究通过预先建立仔猪大肠杆菌性腹泻病理模型,设计10mL/10Kg药物组、5mL/10Kg药物组、2.5mL/10Kg药物组、模型对照组和空白对照组,连续灌服给药7d,观察复方对腹泻仔猪的防治效果。结果表明,各剂量组仔猪临床症状、白细胞计数(WBC)、白球比(A/G)及钠(Na)、钾(K)等电解质指标与空白对照组无显著差异,与模型对照组差异明显(P0.05)。十二指肠组织病理学观察各剂量组没有发现明显病变,模型对照组肠粘膜上皮及腺体坏死脱落,固有层间质中度炎症细胞增多浸润,损伤结构没有恢复,由此说明该复方能有效防治仔猪腹泻。4复方及几味单药对大肠杆菌体外抑菌效果研究试验采用琼脂扩散法和微量二倍稀释法应用复方及几味单药水提取物对猪大肠杆菌进行体外抑菌试验,测定抑菌圈直径和最小抑菌浓度(MIC)。结果表明:黄连、黄芩和复方对大肠杆菌敏感,体外抑菌效果比较好。
[Abstract]:GE Qi compound is a new compound selected independently by the Pharmacy Research Department of Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences. It has a good anti-diarrhea effect according to the dialectical treatment theory of Chinese veterinary medicine. In order to evaluate the effect of the compound, the pre-clinical efficacy evaluation test was carried out, including the effect of the compound on the diarrhea induced by Escherichia coli in mice, and the pathological model of colibacillosis in piglets. Study on the effect of compound on diarrhea caused by Escherichia coli in piglets and the bacteriostatic effect of compound and several single drugs on Escherichia coli in vitro. To provide experimental data for the study of new drugs of GE Qi compound. 1 the effect of compound on Escherichia coli diarrhea in mice was studied. By establishing the pathological model of Escherichia coli diarrhea in mice in advance, the 0.3mL/10g drug group and the 0.2mL/10g drug group were designed. 0.1mL/10g drug group, model control group and blank control group were administered continuously for 7 days to observe the preventive and therapeutic effect of compound on diarrhea mice. The results showed that the average weight gain, hematologic index, spleen and thymus organ index of each dose group were not significantly different from those of the blank control group after administration (P0.05). Histopathological observation of duodenum no obvious pathological changes were found in each dose group. The intestinal villi in the model control group became thicker and thicker than those in the control group. 2 the pathological model of colibacillosis in piglets was studied by designing 5mL/10Kg infection group, 2.5mL/10Kg infection group, 1mL/10Kg infection group and blank control group. The piglets of each infected group were given corresponding 3 脳 109CFU/mL Escherichia coli suspensions through oral cavity for 3 days. The results showed that the average weight gain, bacteriological examination, hematological and electrolyte indexes of piglets in high and medium dose groups were significantly different from those in the control group (P0.05). Therefore, by oral administration of 3 脳 109CFU/mL Escherichia coli suspension, according to the dose of 5mL/10Kg, for 3 days, The effect of compound on diarrhea induced by Escherichia coli in piglets was studied. By establishing the pathological model of Escherichia coli diarrhea in piglets in advance, the 10mL/10Kg drug group and the 5mL/10Kg drug group were designed. 2.5mL/10Kg drug group, model control group and blank control group were administered continuously for 7 days to observe the control effect of compound prescription on diarrhea piglets. The results showed that there were no significant differences in the clinical symptoms, white blood cell count (WBC), white ball ratio (A / G) and sodium, (Na), and potassium (K) between the different dosage groups and the blank control group, but there was significant difference between the two groups (P0.05). Duodenal histopathology observation showed no obvious pathological changes in each dose group. The intestinal mucosal epithelium and glands in the model control group were necrotic and exfoliated, the inflammatory cells in the lamina propria were increased and infiltrated, and the injury structure did not recover. The results show that the compound can effectively prevent and cure piglets diarrhea. (4) the study on the bacteriostatic effect of compound and several single drugs on Escherichia coli in vitro; A study on the bacteriostatic effect of compound and several kinds of single medicine extract on pigs by Agar diffusion method and trace double dilution method Bacteriostasis test of Escherichia coli in vitro, Determination of the diameter and minimum inhibitory concentration of bacteriostasis (MIC). The results showed that Coptis chinensis, Radix Scutellariae and compound were sensitive to Escherichia coli and had better bacteriostatic effect in vitro.
【学位授予单位】：中国农业科学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7
，
本文编号：2316161
[Abstract]:GE Qi compound is a new compound selected independently by the Pharmacy Research Department of Shanghai Veterinary Research Institute of Chinese Academy of Agricultural Sciences. It has a good anti-diarrhea effect according to the dialectical treatment theory of Chinese veterinary medicine. In order to evaluate the effect of the compound, the pre-clinical efficacy evaluation test was carried out, including the effect of the compound on the diarrhea induced by Escherichia coli in mice, and the pathological model of colibacillosis in piglets. Study on the effect of compound on diarrhea caused by Escherichia coli in piglets and the bacteriostatic effect of compound and several single drugs on Escherichia coli in vitro. To provide experimental data for the study of new drugs of GE Qi compound. 1 the effect of compound on Escherichia coli diarrhea in mice was studied. By establishing the pathological model of Escherichia coli diarrhea in mice in advance, the 0.3mL/10g drug group and the 0.2mL/10g drug group were designed. 0.1mL/10g drug group, model control group and blank control group were administered continuously for 7 days to observe the preventive and therapeutic effect of compound on diarrhea mice. The results showed that the average weight gain, hematologic index, spleen and thymus organ index of each dose group were not significantly different from those of the blank control group after administration (P0.05). Histopathological observation of duodenum no obvious pathological changes were found in each dose group. The intestinal villi in the model control group became thicker and thicker than those in the control group. 2 the pathological model of colibacillosis in piglets was studied by designing 5mL/10Kg infection group, 2.5mL/10Kg infection group, 1mL/10Kg infection group and blank control group. The piglets of each infected group were given corresponding 3 脳 109CFU/mL Escherichia coli suspensions through oral cavity for 3 days. The results showed that the average weight gain, bacteriological examination, hematological and electrolyte indexes of piglets in high and medium dose groups were significantly different from those in the control group (P0.05). Therefore, by oral administration of 3 脳 109CFU/mL Escherichia coli suspension, according to the dose of 5mL/10Kg, for 3 days, The effect of compound on diarrhea induced by Escherichia coli in piglets was studied. By establishing the pathological model of Escherichia coli diarrhea in piglets in advance, the 10mL/10Kg drug group and the 5mL/10Kg drug group were designed. 2.5mL/10Kg drug group, model control group and blank control group were administered continuously for 7 days to observe the control effect of compound prescription on diarrhea piglets. The results showed that there were no significant differences in the clinical symptoms, white blood cell count (WBC), white ball ratio (A / G) and sodium, (Na), and potassium (K) between the different dosage groups and the blank control group, but there was significant difference between the two groups (P0.05). Duodenal histopathology observation showed no obvious pathological changes in each dose group. The intestinal mucosal epithelium and glands in the model control group were necrotic and exfoliated, the inflammatory cells in the lamina propria were increased and infiltrated, and the injury structure did not recover. The results show that the compound can effectively prevent and cure piglets diarrhea. (4) the study on the bacteriostatic effect of compound and several single drugs on Escherichia coli in vitro; A study on the bacteriostatic effect of compound and several kinds of single medicine extract on pigs by Agar diffusion method and trace double dilution method Bacteriostasis test of Escherichia coli in vitro, Determination of the diameter and minimum inhibitory concentration of bacteriostasis (MIC). The results showed that Coptis chinensis, Radix Scutellariae and compound were sensitive to Escherichia coli and had better bacteriostatic effect in vitro.
【学位授予单位】：中国农业科学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S853.7
，
本文编号：2316161
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