硫酸安普霉素可溶性粉中非法添加乙酰甲喹的HPLC-PDA检测方法的建立
发布时间：2018-11-09 14:06
【摘要】：为检测硫酸安普霉素可溶性粉中非法添加的乙酰甲喹,以十八烷基硅烷键合硅胶为填充剂,磷酸盐缓冲液(取磷酸二氢钠3.0 g,加水1000 m L使溶解,加三乙胺0.5 m L,用饱和氢氧化钠溶液调节p H值至7.0)-甲醇为流动相,二极管阵列检测器(PDA),建立了HPLC-PDA检测方法,并采用峰纯度检查和光谱相似度检查辅助对照品比对方法,对非法添加药物进行确证。在此液相色谱条件下,乙酰甲喹与其他物质峰分离良好。按外标法以峰面积计算,乙酰甲喹的平均回收率为98.1%,RSD为0.3%。该检测方法简便、准确、可靠,可用于测定硫酸安普霉素可溶性粉中非法添加的乙酰甲喹。
[Abstract]:In order to detect the illegal addition of acetylmethaquine in the soluble powder of ampoumycin sulfate, using octadecyl silane bonded silica gel as filler, phosphate buffer solution (sodium dihydrogen phosphate 3.0 g, water 1000 mL) was added to dissolve, and triethylamine was added to 0.5 mL. Using saturated sodium hydroxide solution to adjust pH to 7.0) -methanol as mobile phase and diode array detector (PDA),) as mobile phase, a HPLC-PDA detection method was established. Confirm the illegal addition of drugs. Under this liquid chromatographic condition, the peak of acetylmethaquine was well separated from other substances. According to the external standard method, the average recovery of acetomequine was 98.1% and RSD was 0.3%. The method is simple, accurate and reliable. It can be used for the determination of acetomequine in the soluble powder of ampouamycin sulfate.
【作者单位】： 中国兽医药品监察所;
【分类号】：S859.79
[Abstract]:In order to detect the illegal addition of acetylmethaquine in the soluble powder of ampoumycin sulfate, using octadecyl silane bonded silica gel as filler, phosphate buffer solution (sodium dihydrogen phosphate 3.0 g, water 1000 mL) was added to dissolve, and triethylamine was added to 0.5 mL. Using saturated sodium hydroxide solution to adjust pH to 7.0) -methanol as mobile phase and diode array detector (PDA),) as mobile phase, a HPLC-PDA detection method was established. Confirm the illegal addition of drugs. Under this liquid chromatographic condition, the peak of acetylmethaquine was well separated from other substances. According to the external standard method, the average recovery of acetomequine was 98.1% and RSD was 0.3%. The method is simple, accurate and reliable. It can be used for the determination of acetomequine in the soluble powder of ampouamycin sulfate.
【作者单位】： 中国兽医药品监察所;
【分类号】：S859.79
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