镰刀菌毒素对断奶仔猪脾脏抗氧化能力和白细胞介素-1β、白细胞介素-6分布和表达的影响

发布时间：2018-11-11 22:17

【摘要】：本试验旨在研究自然霉变饲粮中镰刀菌毒素对断奶仔猪脾脏抗氧化能力和白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)分布和表达的影响。选择35日龄平均体重为(8.45±0.94)kg的健康三元杂交(杜×长×大)断奶仔猪母猪40头,随机分为2组,每组20头。对照组饲喂基础饲粮,镰刀菌毒素组饲喂含镰刀菌毒素[玉米赤霉烯酮(ZEN)0.90 mg/kg;呕吐毒素(DON)1.43mg/kg,烟曲霉毒素(FUM)5.85 mg/kg]的试验饲粮。预试期7d,正试期35d。结果表明:1)与对照组相比,镰刀菌毒素显著降低了断奶仔猪血清和脾脏谷胱甘肽过氧化物酶(GSH-Px)和总超氧化物歧化酶(T-SOD)活性(P0.05),显著升高了丙二醛(MDA)含量(P0.05)。2)镰刀菌毒素使断奶仔猪脾脏白髓区明显变小,红髓区扩张且出现近圆形小空洞,动脉周围淋巴鞘中淋巴细胞数量较少。3)镰刀菌毒素导致断奶仔猪脾脏IL-1β和IL-6阳性细胞主要集中于白髓边缘,且靠近血窦的地方阳性点更多。4)与对照组相比,镰刀菌毒素显著升高了断奶仔猪脾脏IL-1β和IL-6mRNA相对表达量(P0.05)。由此可见,饲粮中镰刀菌毒素显著影响断奶仔猪血清和脾脏抗氧化能力,并通过改变脾脏IL-1β和IL-6的分布和表达,降低脾脏的免疫功能。
[Abstract]:The purpose of this study was to study the effects of Fusarium toxin in naturally moldy diet on the distribution and expression of interleukin-1 尾 (IL-1 尾) and interleukin-6 (IL-6) in spleen of weaned piglets. A total of 40 weaned sows with average body weight of (8.45 卤0.94) kg at 35 days of age were randomly divided into 2 groups (20 pigs per group). The control group was fed with basic diet, and the Fusarium toxin group was fed with Fusarium toxin (DON) 1.43 mg / kg, (FUM) 5.85 mg/kg), and the control group was fed with Fusarium toxin [(ZEN) 0.90 mg/kg;, (DON) 1.43 mg / kg, (FUM) 5.85 mg/kg]. The pretest period was 7 days and the positive test period was 35 days. The results showed that: 1) compared with the control group, Fusarium toxin significantly decreased the activities of glutathione peroxidase (GSH-Px) and total superoxide dismutase (T-SOD) in serum and spleen of weaned piglets (P0.05). The content of malondialdehyde (MDA) was significantly increased (P0.05). 2) Fusarium toxin significantly reduced the white pulp area of spleen, expanded the red pulp area and appeared near circular cavity. The number of lymphocytes in periarterial lymphatic sheath was small. 3) Fusarium toxin caused IL-1 尾 and IL-6 positive cells in spleen of weaned piglets mainly concentrated on the margin of white pulp, and there were more positive spots near the blood sinuses. 4) compared with the control group, there were more positive spots in the spleen of the weaned piglets than those in the control group. Fusarium toxin significantly increased the relative expression of IL-1 尾 and IL-6mRNA in spleen of weaned piglets (P0.05). Therefore, dietary Fusarium toxin significantly affected the antioxidant capacity of serum and spleen of weaned piglets, and decreased the immune function of spleen by changing the distribution and expression of IL-1 尾 and IL-6 in spleen.
【作者单位】：山东农业大学动物科技学院;
【基金】：山东省现代农业产业技术体系生猪创新团队专项资金(SDAIT-08-05)
【分类号】：S858.28

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