绒山羊皮肤毛囊发育不同时期初级、次级毛囊比较转录组学研究

发布时间：2018-11-12 20:15

【摘要】：内蒙古绒山羊具有优良的产绒性状,是宝贵的种质遗传资源,因此研究绒山羊的绒毛发育机理,探寻与绒毛生长相关的重要候选基因就显得尤为重要。本试验采用高通量测序技术,选取3只内蒙古白绒山羊绒毛发育不同时期(生长期11月份、退行期1月份、休止期3月份)体侧部皮肤初级毛囊和次级毛囊各3个RNA样本,等量混合后进行转录组测序,并对结果进行生物信息学分析及qRT-PCR验证。试验结果显示：1、试验分别对3只不同生长时期绒山羊的初级、次级毛囊进行了组间和组内的差异基因筛选,共得到9组差异基因,并对差异基因进行了GO和Pathway分析。GO分析显示：显著富集的条目大多与组织细胞外基质、核糖体结构组成、细胞平移伸长、胞质小核糖体、肌动蛋白及维生素K的代谢过程有关。Pathway分析显示：差异基因主要在核糖体代谢、氨基酸代谢、维生素代谢、硫代谢、新霉素生物合成等功能上影响显著。2、试验对绒山羊不同生长时期初级、次级毛囊组内进行差异基因筛选,发现这些基因都有8种连续的趋势变化,分别是：down down down: up up down: down up down down up up: up down dowm; up down up:down down up: up up up；且在初、次级毛囊组内前三种趋势都较为显著。对这8中趋势的差异基因进行分析研究,将对挖掘绒山羊毛囊(尤其是次级毛囊)周期性生长调控机理提供有力依据。3、试验采用qRT-PCR技术对绒山羊毛囊发育的11月份及3月份的LGR5和SOSTDC1基因的转录组测序结果进行验证。结果显示,待测基因LGR5.SOSTDC1在绒山羊毛囊发育的11月份相对表达量显著高于3月份。这与测序结果基本一致。说明测序结果真实有效。
[Abstract]:Inner Mongolia cashmere goats are valuable germplasm genetic resources, so it is very important to study the mechanism of cashmere development and to search for important candidate genes related to the growth of cashmere in Inner Mongolia Cashmere Goat (Inner Mongolia Cashmere Goat). High throughput sequencing technique was used to select 3 RNA samples of primary hair follicles and secondary hair follicles of three Inner Mongolia white cashmere goats at different stages (growth period November, retreat period January, rest month March). The results were analyzed by bioinformatics and qRT-PCR. The results showed that: 1, 9 groups of differential genes were obtained from the primary and secondary hair follicles of three cashmere goats at different growth stages. GO and Pathway analysis of differentially expressed genes were carried out. GO analysis showed that most of the items significantly enriched were associated with extracellular matrix, ribosomal structure, cell translocation and elongation, and cytoplasmic small ribosome. Pathway analysis showed that differential genes mainly affect ribosomal metabolism, amino acid metabolism, vitamin metabolism, sulfur metabolism, neomycin biosynthesis and other functions. Different genes were screened in the primary and secondary hair follicles of cashmere goats at different growth stages. It was found that all of these genes had eight continuous trend changes, respectively,: down down: up down: down up up: up down dowm;. Up down up: up up; At the beginning, the first three trends in the secondary hair follicle group were significant. The analysis of the differentially expressed genes of these eight trends will provide a powerful basis for digging out the mechanism of periodic growth regulation of cashmere goat hair follicles (especially secondary hair follicles). The transcriptome sequencing of LGR5 and SOSTDC1 genes in wool follicles of cashmere goats in November and March were verified by qRT-PCR technique. The results showed that the relative expression of LGR5.SOSTDC1 in wool follicles of cashmere goats in November was significantly higher than that in March. This is basically consistent with the sequencing results. The results show that the sequencing results are true and effective.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S827

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