乌头驴和三粉驴微卫星遗传多态性分析

发布时间：2018-11-13 09:10

【摘要】：实验旨在了解德州驴中乌头驴和三粉驴2个品系的遗传多态性。利用13个微卫星位点,通过建立多重多色毛细管电泳荧光检测方法,对39头三粉驴和16头乌头驴进行遗传多样性分析,对等位基因数、多态信息含量(PIC)、遗传杂合度和有效等位基因数进行统计分析。结果表明:13对微卫星位点三粉驴PIC指数平均值为0.47,乌头驴PIC指数平均值为0.40,德州驴2个品系PIC总的平均值为0.47。其中乌头驴品系平均等位基因数为3.31,平均观测杂合度为0.27,有效等位基因数为2.11。三粉驴品系平均等位基因数为4.46,平均观测杂合度为0.33,有效等位基因数为2.61。由此可见,三粉驴比乌头驴的遗传多态性高,但2个驴品系的遗传杂合度均偏低,遗传变异程度较小。
[Abstract]:The aim of this study was to investigate the genetic polymorphisms of Aconitum and Sanfen Donkey in Texas Donkey. Using 13 microsatellite loci, a multiplex polychromatic capillary electrophoresis fluorescence detection method was established to analyze the genetic diversity of 39 donkeys and 16 Aconitum donkeys. The alleles and polymorphic information content (PIC),) were analyzed. The genetic heterozygosity and the number of effective alleles were statistically analyzed. The results showed that the average value of PIC index of 13 pairs of microsatellite loci was 0.47, the average of PIC index of Aconitum donkeys was 0.40, and the total average value of PIC of two Texas donkey lines was 0.47. The average number of alleles, observed heterozygosity and effective alleles were 3.31, 0.27 and 2.11 respectively. The average number of alleles, observed heterozygosity and effective alleles were 4.46, 0.33 and 2.61 respectively. It can be seen that the genetic polymorphism of the three donkeys is higher than that of the Aconitum donkeys, but the genetic heterozygosity of the two donkey lines is lower and the degree of genetic variation is smaller.
【作者单位】：山东省农业科学院生物技术研究中心山东省农业科学院应用生命科学实验室胶类中药源性DNA分子鉴定实验室;山东省农业科学院畜牧兽医研究所山东省畜禽疫病防治与繁育重点实验室;山东禹城惠民农业科技有限公司山东省德州黑驴养殖繁育保种基地山东省农业科学院地方驴品种资源保护科研中心;山东东阿国胶堂阿胶药业有限公司;
【基金】：山东省农业重大应用技术创新课题(鲁财农指[2014]38号) 2015山东省高效特色畜牧养殖项目 2015年度禹城市现代农业发展基金高效特色畜牧业示范县项目济南市高校院所自主创新项目(201401264)
【分类号】：S822

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