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阴外动脉灌注乳脂前体物对奶牛产奶量及乳腺内短链脂肪酸摄取规律的影响

发布时间:2018-11-13 11:37
【摘要】:本试验研究了阴外动脉灌注乳脂前体物对奶牛产奶量及乳腺内短链脂肪酸摄取规律的影响。选择8头健康荷斯坦奶牛分为2组,每组4头牛。采用2×2交叉试验设计,2组分别饲喂2种不同粗饲料[苜蓿(MF组)、玉米秸秆(CS组)]的饲粮,饲粮的精粗比为45∶55。试验分为2阶段,每阶段20 d,其中预试期14 d,载体灌注期3 d,乳脂前体物灌注期3 d。在载体灌注期,MF组与CS组均接受载体灌注,分别命名为MFC_1组和CSC组;在乳脂前体物灌注期,MF组继续接受载体灌注(MFC_2组),CS组灌注乳脂前体物(CSF组)。测定产奶量、干物质采食量及乳成分。采集尾动脉和乳静脉血浆测定短链脂肪酸浓度和脂肪酸比例。结果表明:1)在灌注乳脂前体物前,CSC组产奶量、4%乳脂校正乳产量、乳脂产量、乳蛋白产量和产乳脂效率显著低于MFC_1组(P0.05),CSC组尾动脉血浆中短链脂肪酸浓度及短链脂肪酸/长链脂肪酸有低于MFC_1组的趋势(0.05≤P0.10)。2)灌注乳脂前体物后,CSF组产乳脂效率和乳蛋白率显著高于CSC组(P0.05),CSF组产奶量、4%乳脂校正乳产量、乳脂率和乳蛋白率尽管在数值上仍然低于MFC_2组,但组间差异不显著(P0.05),而CSF组产乳脂效率显著高于MFC_2组(P0.05)。3)灌注乳脂前体物后,CSF组尾动脉血浆中乙酸、丙酸以及短链脂肪酸浓度显著高于CSC组(P0.05),且乙酸、丁酸及短链脂肪酸浓度显著高于MFC_2组(P0.05),短链脂肪酸/长链脂肪酸有低于MFC_2组的趋势(0.05≤P0.10)。4)除CSF组丁酸动静脉差有高于CSC组的趋势(0.05≤P0.10)外,CSC组与MFC_1组、CSC组与CSF组、CSF组与MFC_2组乳腺对乙酸、丙酸、丁酸、短链脂肪酸的摄取量、摄取率和动静脉差均无显著差异(P0.05)。结果提示,以玉米秸秆为粗饲料奶牛灌注乳脂前体物可显著增加奶牛的产奶量、乳脂率和乳脂产量,并达到与以苜蓿等为粗饲料相似的水平,且具有较高的产乳脂效率,但对乳腺内短链脂肪酸的摄取量和摄取率无显著的影响。
[Abstract]:This experiment was conducted to study the effect of milk fat precursor infused by external negative artery on milk yield and intake of intramammary short chain fatty acids in dairy cows. Eight healthy Holstein cows were divided into 2 groups with 4 cows in each group. The 2 脳 2 cross experiment design was used. The two groups were fed with two different kinds of forage diet (MF group and CS group) respectively. The ratio of fine to coarse diet was 45: 55. The experiment was divided into two stages, each stage was 20 days, in which the pretest period was 14 days, the carrier perfusion period was 3 days, and the milk precursor perfusion period was 3 days. During the period of carrier perfusion, MF group and CS group received carrier perfusion, named MFC_1 group and CSC group, while MF group continued to receive carrier perfusion (MFC_2 group,), CS group, CSF group). Milk yield, dry matter intake and milk composition were measured. The concentration and proportion of short chain fatty acids were measured in caudal artery and milk vein. The results showed that: 1) the milk yield, corrected milk yield, milk fat yield, milk protein yield and milk fat efficiency of CSC group were significantly lower than those of MFC_1 group (P0.05). The concentrations of short chain fatty acids and short chain fatty acids / long chain fatty acids in caudal artery of CSC group were lower than those in MFC_1 group (0. 05 鈮,

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