一株新亚群禽白血病病毒全基因组序列分析

发布时间：2018-11-15 06:37

【摘要】：为了解外源性禽白血病病毒(ALV)在山东省部分地区肉鸡中的流行状况,采集无菌抗凝血分离血浆接种DF-1细胞、ELISA p27抗原检测以及DNA提取进行PCR扩增等方法,从山东省某地区大型养殖场不同个体养殖场出栏肉鸡群中分离鉴定出1株ALV,命名为FC1505。分析其囊膜糖蛋白gp85氨基酸序列与近年来从地方品种鸡中疑似新亚群ALV-K分离株的相似性最高,相似性均在95%以上,而与其他已知亚群ALV的相似性均低于90%。为了进一步分析该分离株分子特性,对其进行全基因组测序,并与已知亚群ALV分离株序列进行比较。结果表明,FC1505分离株整个基因组中gag、pol和gp37基因相对保守,与ALV参考毒株序列相似性都在90%以上,但均与疑似K亚群的ALV分离株相似性最高;全基因组序列分析进一步说明FC1505属于ALV-K。本研究继我国江苏省和华南地区K亚群ALV报道后,首次从山东地区肉鸡中鉴定到一株ALV-K并完成其全基因组序列分析。
[Abstract]:In order to understand the prevalence of exogenous avian leukemia virus (ALV) in broiler chickens in some areas of Shandong Province, we collected sterile anticoagulant blood samples and inoculated DF-1 cells, detected ELISA p27 antigen, and extracted DNA for PCR amplification. A strain of ALV, named FC1505. was isolated and identified from the flocks of chickens from different individual farms in a large farm in Shandong Province. The amino acid sequence of envelope glycoprotein (gp85) was similar to that of ALV-K isolates of suspected new subgroup from local chickens in recent years, and the similarity was more than 95%, but the similarity with other known subgroups ALV was lower than 90%. In order to further analyze the molecular characteristics of the isolate, the whole genome was sequenced and compared with the known subgroup ALV isolate. The results showed that gag,pol and gp37 genes in the whole genome of FC1505 isolates were relatively conserved, and the sequence similarity between gag,pol and ALV reference strains was more than 90%, but the similarity was the highest with ALV isolates with suspected K subgroup. The whole genome sequence analysis further indicates that FC1505 belongs to ALV-K. After ALV of K subgroup in Jiangsu and South China was reported, a strain of ALV-K was first identified from chicken in Shandong province and its whole genome sequence was analyzed.
【作者单位】：滕州市畜牧局;山东省农业科学院畜牧兽医研究所;山东农业大学动物科技学院;
【基金】：滕州市应用技术研究与开发计划(滕科201505007-02)
【分类号】：S858.31

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