猪流行性腹泻病毒山西分离株全基因组序列分析

发布时间：2018-11-16 08:26

【摘要】：用RT-PCR扩增分离的一株PEDV CH-SXCH11-2015的全基因组序列,测序拼接后,进行了序列分析。CH-SXCH11-2015全长28 038bp比CV777长5bp,与BJ-2011-1同源性最高,为98.9%;与LZC、SM98同源性最低,为96.6%。S基因全长均为4 161bp,突变主要发生在S1区,同源性分析表明,CH-SXCH11-2015核苷酸同源性与BJ-2011-1和CH/ZJJS-Z/2012的最高为98.9%,与SM98同源性最低为93.6%,与CV777、CV777vaccine同源性分别为94.2%和93.8%。CH-SXCH11-2015S基因氨基酸同源性与CH/ZJJS-Z/2012的最高为98.3%,与CV777vaccine、SQ2012同源性最低为91.8%,与CV777的同源性为92.9%。ORF3基因全长均为675bp,无核苷酸插入和缺失,同源性分析表明,CH-SXCH11-2015的ORF3基因与CH/HBQX/10核苷酸同源性最高,为99.3%,与CV777truncated核苷酸同源性最低,为90.6%。CH-SXCH11-2015的ORF3基因推导的氨基酸与(CH/HBQX/10、CH/S、CH/SHH/06、CH/TJ-2012、CH/ZKXH/11、CPF299、GZGY-10、PFF188、PFF285)同源性最高,为98.7%;与CV777truncated毒株最低,为88%。CH-SXCH11-2015的ORF3基因与CV777、attenuated DR13的核苷酸同源性分别为96.6%和97.6%,氨基酸同源性分别为95.1%和97.1%。遗传进化分析表明,CH-SXCH11-2015全基因组、S基因、ORF3基因与2010年以后我国分离的毒株亲缘关系较近,与CV777、2个疫苗株(attenuated DR13、CV777vaccine)的亲缘关系较远。
[Abstract]:The whole genomic sequence of a isolated PEDV CH-SXCH11-2015 was amplified by RT-PCR and sequenced. The total length of CH-SXCH11-2015 28 038bp was 5 BP longer than that of CV777, which was the highest homology with BJ-2011-1, 98.9; The homology with LZC,SM98 was the lowest, the total length of the gene was 4161bp. the mutation mainly occurred in S1 region, and the homology analysis showed that, The nucleotide homology of CH-SXCH11-2015 was 98.9 with BJ-2011-1 and CH/ZJJS-Z/2012, the lowest with SM98, and 93.6 with CV777,. The homology of CV777vaccine was 94.2% and the amino acid homology of 93.8%.CH-SXCH11-2015S gene was 98.3% with CH/ZJJS-Z/2012 and 91.8% with CV777vaccine,SQ2012, respectively. The homology of 92.9%.ORF3 gene with CV777 was 675bp. there was no nucleotide insertion and deletion. Homology analysis showed that the ORF3 gene of CH-SXCH11-2015 had the highest homology with CH/HBQX/10 nucleotide, which was 99.3 BP. The nucleotide homology with CV777truncated is the lowest, the amino acid deduced from the ORF3 gene of 90.6%.CH-SXCH11-2015 and (CH/HBQX/10,CH/S,CH/SHH/06,CH/TJ-2012,CH/ZKXH/11,CPF299,GZGY-10,PFF188,) The homology of PFF285 was the highest (98.7%); The nucleotide homology of ORF3 gene and CV777,attenuated DR13 was 96.6% and 97.6%, respectively, and the amino acid homology was 95.1% and 97.1%, respectively. Genetic evolution analysis showed that the whole genome of CH-SXCH11-2015, S gene and ORF3 gene were closely related to the virus strains isolated from China since 2010, and far related to the two CV777, vaccine strains (attenuated DR13,CV777vaccine).
【作者单位】：山西省农业科学院畜牧兽医研究所;
【基金】：山西省科技攻关项目(20140311020-2-A);山西省科技攻关项目(20150311018-3)
【分类号】：S852.651

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