玉米秸秆饲粮条件下阴外动脉灌注氨基酸混合物对奶牛乳腺内短链脂肪酸摄取规律的影响
发布时间：2018-11-17 16:34
【摘要】：本试验采用动静脉血插管技术,以泌乳中期荷斯坦奶牛为研究对象,研究玉米秸秆饲粮条件下阴外动脉灌注氨基酸(AA)混合物对奶牛产奶性能、尾动脉血和乳静脉血中短链脂肪酸(SCFA)浓度及其比例以及乳腺内SCFA摄取规律的影响。采用2×2交叉试验设计,将体况良好、体重相近、日乳产量为(20.17±1.28)kg的8头经产(2~3胎)荷斯坦奶牛随机分为采食不同饲粮的2组[苜蓿组(MF组)和玉米秸秆组(CS组)],每组4头。2组奶牛饲喂的饲粮精粗比均为45∶55,精饲料组成相同,粗饲料组成不同,MF组粗饲料由苜蓿干草、玉米青贮和羊草组成,CS组以单一的玉米秸秆全部替代MF组饲粮中的粗饲料。试验分为2个阶段,每个阶段20 d,均分为饲粮适应期(预试期)14 d,载体灌注期3 d,正式灌注期3 d。在第1阶段,在载体灌注期,MF组奶牛接受载体灌注(阳性对照组1),CS组奶牛也接受载体灌注(对照组);在正式灌注期,MF组奶牛继续接受载体灌注(阳性对照组2),CS组奶牛接受AA混合物灌注;在第2阶段,将2组动物互换后处理方法同第1阶段。每个正式灌注期的最后2 d采集乳样和血样。结果显示:CS组奶牛阴外动脉灌注AA混合物可显著提高乳蛋白率(P0.05),对乳产量、4%乳脂校正乳(FCM)产量、乳脂率、乳脂产量、乳蛋白产量有一定促进效果,但部分指标仍然显著低于MF组(P0.05)。CS组奶牛阴外动脉灌注AA混合物可趋于显著地提高乳静脉血中乙酸的浓度(P=0.09),显著降低乳腺内乙酸的动静脉差(P0.05),缩小CS组与MF组奶牛在乳腺对乙酸的摄取量和摄取效率方面存在的差距。CS组奶牛阴外动脉灌注AA混合物对提高尾动脉血中乙酸/丙酸、(乙酸+丁酸)/丙酸有一定的促进效果(P0.05)。由此得出,以玉米秸秆为粗饲料的奶牛阴外动脉灌注AA混合物可显著提高乳蛋白率,增加乳静脉血中的乙酸浓度,同时缩小与以苜蓿干草、玉米青贮和羊草为粗饲料的奶牛在乳腺对乙酸的摄取量和摄取效率方面存在的差距。
[Abstract]:In this study, the effects of amino acid (AA) mixture on milk production of Holstein cows in middle lactation period were studied under the condition of corn straw diet by arterial infusion of amino acid (AA). Effects of the concentration and proportion of short-chain fatty acid (SCFA) in caudal blood and mammary vein blood and SCFA uptake in mammary gland. The design of 2 脳 2 cross test shows that the body is in good condition and the weight is similar. Eight Holstein cows with daily milk yield of (20.17 卤1.28) kg were randomly divided into two groups [MF group (MF group) and CS group]. There were 4 cows in each group. The feed ratio of the two groups was 45: 55, the composition of concentrate was the same and the composition of roughage was different. The crude feed of MF group was composed of alfalfa hay, corn silage and Leymus chinensis. In CS group, the crude feed in the diet of MF group was replaced by a single corn straw. The experiment was divided into two stages, 20 days for each stage, 14 days for diet adaptation (pre-test period), 3 days for carrier perfusion and 3 days for formal perfusion. In the first stage, during the period of carrier perfusion, MF cows received carrier perfusion (positive control group 1), CS cows also received carrier perfusion (control group); During the formal perfusion period, the cows in the MF group continued to receive carrier perfusion (the positive control group 2), CS cows received the AA mixture perfusion, and in the second stage, the two groups of animals were exchanged after treatment with the first stage. Milk and blood samples were collected on the last 2 days of each formal perfusion period. The results showed that AA mixture could significantly increase milk protein rate (P0.05) in CS group, which could promote milk yield, 4% milk fat corrected milk (FCM) yield, milk fat yield and milk protein yield. However, some indexes were still significantly lower than those in MF group (P0.05). CS group). The concentration of acetic acid in milk vein blood (P0. 09) and the arteriovenous difference of intramammary acetic acid (P0. 09) were significantly decreased in). CS group (P0.05). To reduce the difference between CS group and MF group in the intake and efficiency of acetic acid in mammary gland. CS group cows were perfused with AA mixture to increase acetic acid / propionic acid in caudal blood. (butyric acid acetate) / propionic acid had certain promoting effect (P0.05). It was concluded that AA mixture infused with cornstalk as forage could significantly increase milk protein rate, increase acetic acid concentration in milk vein blood, and reduce alfalfa hay at the same time. The difference in intake and efficiency of acetic acid in mammary gland between cows with corn silage and Leymus chinensis as forage.
【作者单位】： 内蒙古农业大学动物科学学院;
【基金】：国家奶业“973计划”项目(2011CB1008003)
【分类号】：S823.5
本文编号：2338447
[Abstract]:In this study, the effects of amino acid (AA) mixture on milk production of Holstein cows in middle lactation period were studied under the condition of corn straw diet by arterial infusion of amino acid (AA). Effects of the concentration and proportion of short-chain fatty acid (SCFA) in caudal blood and mammary vein blood and SCFA uptake in mammary gland. The design of 2 脳 2 cross test shows that the body is in good condition and the weight is similar. Eight Holstein cows with daily milk yield of (20.17 卤1.28) kg were randomly divided into two groups [MF group (MF group) and CS group]. There were 4 cows in each group. The feed ratio of the two groups was 45: 55, the composition of concentrate was the same and the composition of roughage was different. The crude feed of MF group was composed of alfalfa hay, corn silage and Leymus chinensis. In CS group, the crude feed in the diet of MF group was replaced by a single corn straw. The experiment was divided into two stages, 20 days for each stage, 14 days for diet adaptation (pre-test period), 3 days for carrier perfusion and 3 days for formal perfusion. In the first stage, during the period of carrier perfusion, MF cows received carrier perfusion (positive control group 1), CS cows also received carrier perfusion (control group); During the formal perfusion period, the cows in the MF group continued to receive carrier perfusion (the positive control group 2), CS cows received the AA mixture perfusion, and in the second stage, the two groups of animals were exchanged after treatment with the first stage. Milk and blood samples were collected on the last 2 days of each formal perfusion period. The results showed that AA mixture could significantly increase milk protein rate (P0.05) in CS group, which could promote milk yield, 4% milk fat corrected milk (FCM) yield, milk fat yield and milk protein yield. However, some indexes were still significantly lower than those in MF group (P0.05). CS group). The concentration of acetic acid in milk vein blood (P0. 09) and the arteriovenous difference of intramammary acetic acid (P0. 09) were significantly decreased in). CS group (P0.05). To reduce the difference between CS group and MF group in the intake and efficiency of acetic acid in mammary gland. CS group cows were perfused with AA mixture to increase acetic acid / propionic acid in caudal blood. (butyric acid acetate) / propionic acid had certain promoting effect (P0.05). It was concluded that AA mixture infused with cornstalk as forage could significantly increase milk protein rate, increase acetic acid concentration in milk vein blood, and reduce alfalfa hay at the same time. The difference in intake and efficiency of acetic acid in mammary gland between cows with corn silage and Leymus chinensis as forage.
【作者单位】： 内蒙古农业大学动物科学学院;
【基金】：国家奶业“973计划”项目(2011CB1008003)
【分类号】：S823.5
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